Protein Info for UW163_RS20470 in Ralstonia solanacearum UW163

Annotation: fructosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01322: sucrose-6-phosphate hydrolase
  • amino acids 11 to 445 (435 residues), 464.2 bits, E=2.1e-143
• PF00251: Glyco_hydro_32N
  • amino acids 21 to 320 (300 residues), 325.1 bits, E=5.7e-101
• PF08244: Glyco_hydro_32C
  • amino acids 335 to 469 (135 residues), 57.8 bits, E=1.4e-19

Best Hits

KEGG orthology group: K01193, beta-fructofuranosidase [EC: 3.2.1.26] (inferred from 87% identity to rsl:RPSI07_mp1101)

Predicted SEED Role

"Sucrose-6-phosphate hydrolase (EC 3.2.1.B3)" (EC 3.2.1.B3)

MetaCyc Pathways

• sucrose degradation III (sucrose invertase) (4/4 steps found)
• sucrose degradation V (sucrose α-glucosidase) (3/5 steps found)
• inulin degradation (1/3 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Starch and sucrose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.26 or 3.2.1.B3

Protein Sequence (476 amino acids)

>UW163_RS20470 fructosidase (Ralstonia solanacearum UW163)
MLDDVLAPLDVIEDPWRQAYHLCPPLGLLSDPNGLTFWRGAYHVFYQWNPHGCGHENKHW
AHVRSTDLIHWEWLPPALAPDAPYDEDGCYSGCAVELGGDLFLMYSGNVRSADGGRESYQ
CLARSRDGVGFEKLGPVINGALPGYTGHFRDPKAWRHGDRWYAVLGAQRNDLTGTVLLLE
SPDLCEWHSVGELIQPAPPCYMYECPDLFTLDGRTVLVCCQQVLEASADGTRRSDVAGYM
LGWVDLERARFPHSGFHKLDQGFDFYAPQTLLGPDGRRLLIGWMGLPVQPQTPSVDSGWT
HCLTLPRELTLEGDQLMQRPARELARLRSMAIRVPDVDMDANTAFVLPQPDGGAWELDLS
IRAARGADWVLHLFDSEQQSLSLVFDGAGGRLTLQRHCPAAGQLHESRTCEMGGAVTRLQ
VFIDRSSAEIFVNDGVAVFTTRCYPTARPEAPRLLARQALTLHDIHVWPLEDRLTR