Protein Info for UW163_RS20370 in Ralstonia solanacearum UW163

Annotation: formate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01701: oxidoreductase alpha (molybdopterin) subunit
  • amino acids 13 to 766 (754 residues), 990.5 bits, E=2.4e-302
• PF00384: Molybdopterin
  • amino acids 109 to 545 (437 residues), 91.9 bits, E=4.3e-30
• PF01568: Molydop_binding
  • amino acids 653 to 759 (107 residues), 27.1 bits, E=3.6e-10

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 94% identity to rsl:RPSI07_mp1081)

Predicted SEED Role

"Putative formate dehydrogenase oxidoreductase protein" in subsystem Formate hydrogenase

MetaCyc Pathways

• formate oxidation to CO₂ (1/1 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• oxalate degradation VI (1/4 steps found)
• oxalate degradation III (1/5 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Protein Sequence (783 amino acids)

>UW163_RS20370 formate dehydrogenase (Ralstonia solanacearum UW163)
MKSQKIQFYRHPAGGWGALKSVAHQLLTHGIAAKGAKTLLSANQPDGFDCPGCAWPDRDH
ASTFEFCENGVKAVAAEATARRTTPEFFAQHTVRELAGWSDFALEDQGRLTHPMVYDAAT
DKYQPIAWDDAFALIAQHLRALPDPNQAIFYTSGRTSNEAAFLYQLFVRAYGTNNFPDCS
NMCHEPSGTGMRSSIGVGKGTVTLDDFTKADAIFIFGQNPGTNHPRMLGELRDASRRGAR
IVSFNPLRERGLERFADPQSKIEMLTLGSTRISTEYHQVRIGGDLATIKGIIKHVIERDD
AARGRGRSDDNRAIIDHDFVARHTSGYDAFAADVRAESWATIEAESGLPEAEIRQAGDTY
IQAGSVIACWGMGITQHKHSVATIQMIVNLLLLRGNIGRPGAGACPVRGHSNVQGDRTMG
IYEKPAPAFLDRLEKVFGFSVPRAHGYDTVGAIEAMRGGAARVFFAMGGNFAAATPDTAA
TWAGLRQCDLTVHVTTKLNRSHIVHGKAALILPCLGRTEIDQQAGGAQGVTVEDSMSMVH
MSAGINPPASPHLLSEPAIVARLAEATLPGGAVPWRWLVEDYDRIRERIEAVFDDFAGFN
EKIRVPGGFRLRNTASERVWNTSSGKAEFHAHAVPTDTPVHRARARHADATVFTLATVRS
HDQYNTTIYGMDDRYRGVFGQRRVVFANAEDLRAIGMKDGDGVDLLTVSEDGVTRRADGF
RLVAYDIPRGCLAAYYPETNPLVPLSAVADQARTPTSKSIPVMLVPSQATPRTEADTAAT
AEA