Protein Info for UW163_RS20185 in Ralstonia solanacearum UW163

Annotation: NAD-dependent succinate-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 21 to 483 (463 residues), 568.1 bits, E=6.1e-175
• TIGR01780: succinate-semialdehyde dehydrogenase
  • amino acids 31 to 481 (451 residues), 677.4 bits, E=4.7e-208

Best Hits

Swiss-Prot: 54% identical to SLAD_PSEPU: 3-sulfolactaldehyde dehydrogenase (PpSQ1_00395) from Pseudomonas putida

KEGG orthology group: K00135, succinate-semialdehyde dehydrogenase (NADP+) [EC: 1.2.1.16] (inferred from 94% identity to rsl:RPSI07_mp1031)

MetaCyc: 53% identical to succinate-semialdehyde dehydrogenase (NAD+) (Alicycliphilus denitrificans)
Succinate-semialdehyde dehydrogenase. [EC: 1.2.1.24]

Predicted SEED Role

"Aldehyde dehydrogenase B (EC 1.2.1.22)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Methylglyoxal Metabolism (EC 1.2.1.22)

MetaCyc Pathways

• 4-aminobutanoate degradation I (2/2 steps found)
• 4-aminobutanoate degradation III (2/2 steps found)
• GABA shunt I (3/4 steps found)
• GABA shunt II (3/4 steps found)
• methylglyoxal degradation IV (2/3 steps found)
• methylglyoxal degradation V (2/3 steps found)
• superpathway of 4-aminobutanoate degradation (2/3 steps found)
• L-lactaldehyde degradation (aerobic) (1/2 steps found)
• superpathway of methylglyoxal degradation (5/8 steps found)
• 4-aminobutanoate degradation IV (1/3 steps found)
• N-methylpyrrolidone degradation (1/3 steps found)
• superpathway of L-arginine and L-ornithine degradation (8/13 steps found)
• lactate biosynthesis (archaea) (2/5 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (6/11 steps found)
• L-rhamnose degradation II (2/8 steps found)
• superpathway of fucose and rhamnose degradation (3/12 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Glutamate metabolism
• Pyruvate metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.16, 1.2.1.22

Use Curated BLAST to search for 1.2.1.16 or 1.2.1.22 or 1.2.1.24

Protein Sequence (489 amino acids)

>UW163_RS20185 NAD-dependent succinate-semialdehyde dehydrogenase (Ralstonia solanacearum UW163)
MPLTLDRTDLVRSANYIAGTWTAAAGPLLDVTDPATGELITRVPDSGAAEARAALDAAQA
AFAAWRKVPAKLRAQIIKRWNDLVLAHQDDLGKLISREQGKPLAEGKGEVAYAASYIEWF
GEEATRMNGETIPAPVPGRRMFALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVC
KPAEDTPLTSLALVRLAEEAGVPAGVLNIVTASRERTPEVVDVWLDDPRVRKITFTGSTP
VGKHLARRSAETLKKLSLELGGNAPFIVFEDADLDAAVDGFMAAKFRNGGQTCVCPNRVF
VHDAVHDAFAARLAARVEALKVGPASDPASQIGPMINARAVEKIERHVQDAVARGARVLT
GGRRLTELGPNYYAPTVLTHADATMACACEETFGPVAPLTRFTTEAEVIAAANDTPFGLA
AYFYSQDVRRIWRVADQLETGIVGVNEGAVASEAAPFGGVKESGYGREGSVHGLDDYLHT
KYVCQGQLD