Protein Info for UW163_RS19775 in Ralstonia solanacearum UW163

Annotation: polyketide synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00109: ketoacyl-synt
  • amino acids 52 to 293 (242 residues), 295.4 bits, E=2.2e-91
  • amino acids 1540 to 1785 (246 residues), 252 bits, E=3.8e-78
• PF02801: Ketoacyl-synt_C
  • amino acids 302 to 417 (116 residues), 123.6 bits, see alignment (E = 2.5e-39)
  • amino acids 1793 to 1908 (116 residues), 108.6 bits, see alignment (E = 1.1e-34)
• PF16197: KAsynt_C_assoc
  • amino acids 421 to 527 (107 residues), 49.1 bits, see alignment (E = 3.9e-16)
  • amino acids 1914 to 2041 (128 residues), 38.7 bits, 6.8e-13
• PF21089: PKS_DH_N
  • amino acids 639 to 738 (100 residues), 79.8 bits, see alignment (E = 7.5e-26)
• PF14765: PS-DH
  • amino acids 768 to 927 (160 residues), 82.8 bits, 1.2e-26
• PF08659: KR
  • amino acids 1117 to 1303 (187 residues), 176.6 bits, 2.7e-55
• PF00106: adh_short
  • amino acids 1119 to 1277 (159 residues), 40.8 bits, 1e-13
• PF13561: adh_short_C2
  • amino acids 1127 to 1276 (150 residues), 35.5 bits, 4.6e-12
• PF00550: PP-binding
  • amino acids 1419 to 1480 (62 residues), 55.7 bits, see alignment (E = 2.7e-18)
  • amino acids 2206 to 2267 (62 residues), 44.6 bits, see alignment (E = 7.7e-15)
• PF00975: Thioesterase
  • amino acids 2310 to 2584 (275 residues), 116.2 bits, E=1.4e-36

Predicted SEED Role

"Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)" in subsystem Fatty Acid Biosynthesis FASII or mycolic acid synthesis (EC 2.3.1.39)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (50/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (14/16 steps found)
• fatty acid biosynthesis initiation (type II) (3/3 steps found)
• superpathway of fatty acid biosynthesis initiation (4/5 steps found)
• fatty acid biosynthesis initiation (mitochondria) (2/4 steps found)
• fatty acid biosynthesis initiation (plant mitochondria) (1/4 steps found)
• mycobactin biosynthesis (3/11 steps found)
• pederin biosynthesis (2/14 steps found)
• bryostatin biosynthesis (2/19 steps found)
• mupirocin biosynthesis (1/26 steps found)
• corallopyronin A biosynthesis (2/30 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.39

Use Curated BLAST to search for 2.3.1.39

Protein Sequence (2600 amino acids)

>UW163_RS19775 polyketide synthase (Ralstonia solanacearum UW163)
MKTTTANEANDISLPNQRRAGQRQLAPQRTRRQIGADPVREPGSQPAPETGKVAIVGFSG
RFPGADNPQAFWQLLVEGRSAIRAFPYWDLPGIAERRHLRETFACPGGFIQDPTAFDADF
FKISAREAEAMDPQHRLVLEQTWAAFEDAGICPRSLRGSDTGVYIGVSSADYSGLLARAA
AGAHDATGNAHSIIANRISYLFDFHGPSAPVDTACSSSLVAVHRAAQSILRGDCTVAVAG
GVNLCFEPMVFIGAAKTGMLSPGGRCKTFAADADGYVRGEGVGVLILKNYRQALADGDRI
VATIIGSAENHGGHANSLTAPNARAQADLIKQACRGIDIDSIGYIEVHGTGTRLGDPIEI
SGLKQAFAELGGSGTQRCYLGSVKTNIGHLESAAGIAGVIKTLLVLKHGHLPQSLHSEPR
NPYIDLAGSPFEVLREGRAWPMQSGSHGGSRPRRAGVSSFGFGGANAHVVLEAAEPTPGI
TAQGIAATIFPLSARTPAALRSRVEQLIGCIEQQPRPALPDLAASLQLGREAMACRLAIA
ADSRDSLLAQLRLELVDVDRQGGAPAGDMAADAAPRALQQHDRDTLLALWRGGADVDWRQ
LYPDGRAQRIPLPTYPFSRDHYWISDAPVSERAGEPTLHPLVQRNTSDLAGQRFSSRFDG
NEFFFDEHRVNALKILPGVAYLEMARAAIALSLAGDEAPVRLKHIAWRQPMRHDGGTLTI
DIELAQQDNGEIRFEMLSAAANGPSYCHGVALIGTPGPLPPIDLPAIAAATTEQRLAAQQ
CYRIFSEMGLDYGDRFRGIRALQAGNGQVLAHLASLGGAADTGACSEPMRLPPGLLDSAL
QAALGLALLDPAQPAAPRQPLVPFALDAVVFGDLSLLGDDCHAWVRYAADHQPGRINRLD
VDLCDAQGRVALRLLGFSSKRLERGQAEPVVMMPVWRDQALRGDAERHYRRQLILLAGHP
HMAPNTLADHCRALAADDRQIDIERLPDVAEDPAVALQSLAGFLIRRLKACGSDSLVQLV
LPDDGEGQHPLAQVFASLPALLKTASNEGLIAAAQSIEVPGVDSPAQLAAHLIDSSRRDD
ERVRYLGGPGGERSRQVASWRGTVPVAAATAPWRAGGVYLLTGGTGGLGLLLAETIAREA
PGARLILVSRSAGQLPPQTTERIADIQALGAEVRLEVLDVADQSAVDALIARCEQAFSGI
HGIFHCAGLTRDSRLDNKSEADLAAVLAPKVAGTLNLDRASAHLALDCFVLFSSVSAVIG
NPGQGDYALANAFMDAFAGWRHRQATAGHRHGRTLAVNWPLWRNGGMRIDPQRERWMAEQ
TGIQPLSDQGGLELLRRALADTGAPERLMVLEGDAARIRAFVAAQWHEPVAAMFDAPEDA
APSATPVARVPIEPPPTVASAVGESTGSDLVEPAIHHFKQLLSKALKRPAERIDSDGTFE
RYGVDSIILTELTTRLEQHFGSLPNTLLYEYQTVRELSAYFAKHHREALQRLVLGDPANR
RPSVGHAVRVEAAEPPPQRKRRRLNPQRNASPAAGPCHDLAIIGLSGRYPGAEDLETFWS
RLAGGEDLVGEVPAQRWDHQAYFDGQRARLDKTYCKWGGFLEGIEDFDPLFFNLSPREAA
VVNPNDRLFLETCWNLLEGAGLTRQRLKQQYRQQVGVFVGVMYQQYQAFETDLVRASLVS
VASHSAIANRVSYFFDFQGPSLAIDTMCSSSISAIHAAGQALRNGDCRLAIAGGVNLTLH
PKKYIGLSVGRVLGSHAGSRSFADGDGYLPAEGVGAVLLKTLADAERDGDRILAVIKSTA
TNHGGHTHGFSVPSAKAQAELIDGHFKRAGIDPRTISYVESAANGSAMGDAVELSALNRV
FGQAGVAARSCAIGSVKSNIGHAEAASGMSQLSKLVLQLQHRRLVPSLLLGPINPKLDLD
NSPFVLQRELADWPRPVVEIDGERRPCPRRASLSAFGAGGSNAHLIIEEYPGQEAAPGRP
AAGAAQVEDGIRYIVPMSAKSAGALHKGVARLARHLETHPDLRLNDLAYTLQTGREAMAW
RLALLVSRMDELRTVLAGILRVLDQGEGPTALETLDLPLCFGNVDAVPAAMASLLSGPGA
DALSRSLIAADARQQLALLWTQGGEVPWDTLAENTSAGHGARIVPLPTYAFDKKRYWLEP
SGTPAAPRDSAMAADTAPQAPDRALATATDRHAGNDEATAGDHGLRLLAGLLGMQPQELS
PAKPLSDYGADSIVMAQLAMQLQAELDPGLTLTDIESCVTVRDLLAVLTERGHRTGPGGQ
ALPAEISKLFTRPAVRFLELQRLNRVDRGRPVFWFHGALGGCEIYQELARRIGRPFFGLQ
ARGWMTSRQPLQGIQAMAAYYVQAIQAIQSHGPYDLGGYSLGGMLAYEVTRQLQELGETV
ATLVMLDSPDVTGERTARLADKTRLLQTVNMALQMTIGETPERFAEVLIHRDAIDLSLPQ
PEYRQRLTALARQRGLKMSDDRLLQLMEQVTGLQQAYQVEHYRILPLPNAGDVACYYFRN
ASGLWMGDLEPYFSTDSREFAAFNHADYWQTWQRLLPNFEMIEVASSNHMTLLTEPGVFE
VIAKFCASLYAETVPEDTVG