Protein Info for UW163_RS18695 in Ralstonia solanacearum UW163

Annotation: HoxN/HupN/NixA family nickel/cobalt transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 357 transmembrane" amino acids 21 to 42 (22 residues), see Phobius details amino acids 48 to 66 (19 residues), see Phobius details amino acids 87 to 109 (23 residues), see Phobius details amino acids 128 to 151 (24 residues), see Phobius details amino acids 197 to 220 (24 residues), see Phobius details amino acids 234 to 261 (28 residues), see Phobius details amino acids 271 to 296 (26 residues), see Phobius details amino acids 317 to 339 (23 residues), see Phobius details PF03824: NicO" amino acids 49 to 336 (288 residues), 321.6 bits, see alignment E=2.4e-100 TIGR00802: transition metal uptake transporter, Ni2+-Co2+ transporter (NiCoT) family" amino acids 52 to 332 (281 residues), 393 bits, see alignment E=4.1e-122

Best Hits

Swiss-Prot: 55% identical to HOXN_CUPNH: High-affinity nickel transport protein (hoxN) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K07241, high-affinity nickel-transport protein (inferred from 94% identity to rsl:RPSI07_mp0512)

Predicted SEED Role

"HoxN/HupN/NixA family cobalt transporter" in subsystem Coenzyme B12 biosynthesis or Transport of Nickel and Cobalt

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (357 amino acids)

>UW163_RS18695 HoxN/HupN/NixA family nickel/cobalt transporter (Ralstonia solanacearum UW163)
MASPLSPPRLRHLIAQHRAPISQLLLLLVANLAAWAWAFAAFAGHPELLGTALLAYLFGL
RHAVDADHIAAIDNVVRKLMQQGKRPFSVGFFFSIGHSALVVLGVAIIVRATSALNDRID
TFKAIGGMISTGISAFFLLTIAVTNLVILVGTWKHFRRIRSGATLAEADLDMLTAGNGLL
ARLFRPMFRLVTKSWHMAPLGFLFGLGFDTATEIGLFSVAAGHASEGIPPSAVMVFPVLF
AAGMALIDTLDSVLMVGAYGWAFVNPVRKLWYNLTITSVSVLIAFGIGGIEALGLLGRQL
KLNGGFWDQVAALNEGLAHFGYLMIGIFLLAWAVSACIYKWKGYDSLPHPAQGAPLP