Protein Info for UW163_RS17925 in Ralstonia solanacearum UW163

Annotation: isopenicillin N synthase family oxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 PF14226: DIOX_N" amino acids 16 to 122 (107 residues), 55.3 bits, see alignment E=1e-18 PF03171: 2OG-FeII_Oxy" amino acids 169 to 288 (120 residues), 51.9 bits, see alignment E=8.4e-18

Best Hits

Swiss-Prot: 85% identical to EFE_RALSO: 2-oxoglutarate-dependent ethylene/succinate-forming enzyme (efe) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K06892, (no description) (inferred from 88% identity to rsl:RPSI07_mp1626)

MetaCyc: 63% identical to 2-oxoglutarate-dependent ethylene/succinate-forming enzyme (Pseudomonas savastanoi pv. phaseolicola)
RXN-12536 [EC: 1.14.20.7]; RXN-14542 [EC: 1.14.20.7, 1.14.11.41]; 1.13.12.19,1.14.11.- [EC: 1.14.20.7, 1.14.11.41, 1.13.12.19]

Predicted SEED Role

"small molecule metabolism"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.12.19 or 1.14.11.41 or 1.14.20.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (347 amino acids)

>UW163_RS17925 isopenicillin N synthase family oxygenase (Ralstonia solanacearum UW163)
MTGLTTFHLPERILHSEAHRQLGQDMVAAWRADGIFQIALSTPQQHTTDEAFAQSRRFFE
LDFETKRRHVSELTYSGYIASREEITAGEADYSEIFTICPDIGMDDVRVREGWPCHGPVP
WPGTAYRDRMTDFTGMLGAFGERLLQLTALGLGLDDMETFTRLTRDGWHHMRVLRFPTVQ
SSENARGIGAHTDYGLLVIAAQDDVGGLYVRPPIAGERRNRNWLPSESTAGMFEHDDGWT
FIKPEPAVLTVFPGDFLQFLTGGHLMSTPHKVRLNTRERFAMAYFHEPNFDAWVEPLKAD
ADTDVAPIHYGTHFTNMFMRCYPKRITTRRIEEQGLLDRLPALGEVA