Protein Info for UW163_RS17165 in Ralstonia solanacearum UW163

Annotation: peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 490 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR02037: peptidase Do" amino acids 59 to 485 (427 residues), 509.5 bits, see alignment E=4.1e-157 PF00089: Trypsin" amino acids 121 to 276 (156 residues), 65 bits, see alignment E=3.1e-21 PF13365: Trypsin_2" amino acids 122 to 255 (134 residues), 131.4 bits, see alignment E=1.5e-41 PF00595: PDZ" amino acids 290 to 372 (83 residues), 36.3 bits, see alignment E=2.1e-12 amino acids 405 to 477 (73 residues), 32.2 bits, see alignment E=4e-11 PF13180: PDZ_2" amino acids 294 to 385 (92 residues), 65.8 bits, see alignment E=1.3e-21 amino acids 404 to 483 (80 residues), 40.4 bits, see alignment E=1.1e-13 PF17820: PDZ_6" amino acids 321 to 360 (40 residues), 38.7 bits, see alignment 2.4e-13 amino acids 433 to 478 (46 residues), 50.3 bits, see alignment 6e-17

Best Hits

Swiss-Prot: 52% identical to DEGPL_PSEPW: Probable periplasmic serine endoprotease DegP-like (PputW619_1070) from Pseudomonas putida (strain W619)

KEGG orthology group: K01362, [EC: 3.4.21.-] (inferred from 97% identity to rsl:RPSI07_mp1656)

Predicted SEED Role

"macromolecule metabolism; macromolecule degradation; degradation of proteins, peptides, glycopeptides"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (490 amino acids)

>UW163_RS17165 peptidase (Ralstonia solanacearum UW163)
MTRQTMARSAVGLAAVLAVAGGYAYLQKDVISRAVAAPMAASATAPASTAAVAVAAPMDF
SGIVERYGPAVVNISTTAHAQRTSIQGLPPGMSPDDPFAEFFKHFMPQMPQQQGDQVVKG
LGSGFIVSSDGLILTNAHVVDGAQEVNVKLTDRREFKAKVLGVDKQSDVAVLRIAAGNLP
TVQIGNPAGTKVGEPVLAIGSPYGFENTVTAGIVSAKSRSLPDDTYVPFIQTDVAVNPGN
SGGPLFNQRGEVIGINSQIYSQTGGYQGLSFAVPIDVAMKVEQQLVATGKVTRGRLGISV
QEVDQLLADSFNLPKPEGALVNAVEKDGPAAKAGLQPGDVILQIGNAHIGHSGDLPEQVA
EIKPGTTVPLQIIRQGRPTTLSVTVGEAKDAKVAANASAAPDKGRLGLAVRPLQPEEKRQ
SGLPGGLVVMDSSGPAAKAGIQPGDVILSLNGTPVSSAQELRTLVDRAGKHVALLVQRDD
AKIFVPLDLG