Protein Info for UW163_RS16045 in Ralstonia solanacearum UW163

Annotation: histidinol-phosphate transaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 91% identical to HIS82_RALSO: Histidinol-phosphate aminotransferase 2 (hisC2) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00817, histidinol-phosphate aminotransferase [EC: 2.6.1.9] (inferred from 96% identity to rsc:RCFBP_20576)

MetaCyc: 43% identical to histidinol-phosphate aminotransferase; tyrosine/phenylalanine aminotransferase (promiscuous) (Bacillus subtilis subtilis 168)
Histidinol-phosphate transaminase. [EC: 2.6.1.9]; Aspartate transaminase. [EC: 2.6.1.9, 2.6.1.1, 2.6.1.27, 2.6.1.5, 2.6.1.57]; 2.6.1.1,2.6.1.57,2.6.1.27 [EC: 2.6.1.9, 2.6.1.1, 2.6.1.27, 2.6.1.5, 2.6.1.57]

Predicted SEED Role

"Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57)" in subsystem Phenylalanine and Tyrosine Branches from Chorismate (EC 2.6.1.57)

MetaCyc Pathways

superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
superpathway of aromatic amino acid biosynthesis (18/18 steps found)
aspartate superpathway (23/25 steps found)
superpathway of L-isoleucine biosynthesis I (13/13 steps found)
superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
L-histidine biosynthesis (10/10 steps found)
superpathway of L-phenylalanine biosynthesis (10/10 steps found)
superpathway of L-tyrosine biosynthesis (10/10 steps found)
anaerobic energy metabolism (invertebrates, cytosol) (7/7 steps found)
superpathway of L-threonine biosynthesis (6/6 steps found)
L-tyrosine degradation I (5/5 steps found)
L-phenylalanine biosynthesis I (3/3 steps found)
L-phenylalanine degradation II (anaerobic) (3/3 steps found)
L-tyrosine biosynthesis I (3/3 steps found)
superpathway of chorismate metabolism (43/59 steps found)
TCA cycle VIII (Chlamydia) (5/6 steps found)
L-phenylalanine biosynthesis III (cytosolic, plants) (2/2 steps found)
malate/L-aspartate shuttle pathway (2/2 steps found)
superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
3-(4-hydroxyphenyl)pyruvate biosynthesis (1/1 steps found)
L-aspartate biosynthesis (1/1 steps found)
L-aspartate degradation I (1/1 steps found)
L-phenylalanine biosynthesis II (3/4 steps found)
L-phenylalanine degradation III (3/4 steps found)
L-tyrosine biosynthesis III (3/4 steps found)
L-asparagine degradation III (mammalian) (2/3 steps found)
superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
L-glutamate degradation II (1/2 steps found)
L-tryptophan degradation IV (via indole-3-lactate) (1/2 steps found)
L-tyrosine degradation II (1/2 steps found)
atromentin biosynthesis (1/2 steps found)
superpathway of L-phenylalanine and L-tyrosine biosynthesis (3/5 steps found)
L-tyrosine biosynthesis II (2/4 steps found)
L-tyrosine degradation III (2/4 steps found)
superpathway of L-aspartate and L-asparagine biosynthesis (2/4 steps found)
C4 photosynthetic carbon assimilation cycle, NAD-ME type (7/11 steps found)
(R)-cysteate degradation (1/3 steps found)
L-tyrosine degradation IV (to 4-methylphenol) (1/3 steps found)
indole-3-acetate biosynthesis VI (bacteria) (1/3 steps found)
sulfolactate degradation III (1/3 steps found)
L-phenylalanine degradation IV (mammalian, via side chain) (5/9 steps found)
superpathway of plastoquinol biosynthesis (2/5 steps found)
L-tryptophan degradation VIII (to tryptophol) (1/4 steps found)
C4 photosynthetic carbon assimilation cycle, PEPCK type (8/14 steps found)
superpathway of sulfolactate degradation (2/6 steps found)
4-hydroxybenzoate biosynthesis I (eukaryotes) (1/5 steps found)
L-phenylalanine degradation VI (reductive Stickland reaction) (1/5 steps found)
L-tryptophan degradation XIII (reductive Stickland reaction) (1/5 steps found)
L-tyrosine degradation V (reductive Stickland reaction) (1/5 steps found)
coenzyme M biosynthesis II (1/6 steps found)
rosmarinic acid biosynthesis I (2/10 steps found)
indole-3-acetate biosynthesis II (3/12 steps found)
(S)-reticuline biosynthesis I (2/11 steps found)
tropane alkaloids biosynthesis (1/11 steps found)
superpathway of hyoscyamine (atropine) and scopolamine biosynthesis (4/16 steps found)
superpathway of rosmarinic acid biosynthesis (2/14 steps found)
anaerobic aromatic compound degradation (Thauera aromatica) (6/27 steps found)
Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.1, 2.6.1.57, 2.6.1.9

Use Curated BLAST to search for 2.6.1.1 or 2.6.1.27 or 2.6.1.5 or 2.6.1.57 or 2.6.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (370 amino acids)

>UW163_RS16045 histidinol-phosphate transaminase (Ralstonia solanacearum UW163)
MSLQFGPEYVRAIAPYVAGKPISEVAREFGLDASRIVKLASNENPLGMPVSAKAAIDELA
RYPDANGFSLKAALHAKFGVPEAWITLGNGSNDILELAARALVAPGQGVIYAQHSFAVYA
LAAQEVGARAIEVPARDYGHDLEAMAAAITPDTRLIYIANPNNPTGTFLPADAIAAFLAR
VPPTVVVVLDEAYNEFLAPGQQYDSVAWVRRYPNLLVSRTFSKAYGLAGLRVGYGIAQPQ
LADLLNRIRQPFNVNSLAQAAAVAALNDTEFLRRSAELNAAGYAQLTQAFGRLGLEYVPS
WGNFVLVRVGDDPGAGARVNLALLKQGVIVRPVGNYGLPQWLRISIGLPEENAACIAALE
SALAQAKVVA