Protein Info for UW163_RS15035 in Ralstonia solanacearum UW163

Annotation: amino acid permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 transmembrane" amino acids 31 to 53 (23 residues), see Phobius details amino acids 58 to 80 (23 residues), see Phobius details amino acids 90 to 111 (22 residues), see Phobius details amino acids 157 to 176 (20 residues), see Phobius details amino acids 186 to 205 (20 residues), see Phobius details amino acids 217 to 239 (23 residues), see Phobius details amino acids 255 to 281 (27 residues), see Phobius details amino acids 302 to 325 (24 residues), see Phobius details amino acids 351 to 373 (23 residues), see Phobius details amino acids 379 to 398 (20 residues), see Phobius details amino acids 410 to 429 (20 residues), see Phobius details amino acids 435 to 454 (20 residues), see Phobius details PF13520: AA_permease_2" amino acids 26 to 430 (405 residues), 215.9 bits, see alignment E=1.5e-67 PF00324: AA_permease" amino acids 31 to 444 (414 residues), 141.5 bits, see alignment E=5.2e-45 PF13906: AA_permease_C" amino acids 409 to 458 (50 residues), 65.5 bits, see alignment 5.4e-22

Best Hits

Swiss-Prot: 64% identical to YHDG_BACSU: Uncharacterized amino acid permease YhdG (yhdG) from Bacillus subtilis (strain 168)

KEGG orthology group: K03294, basic amino acid/polyamine antiporter, APA family (inferred from 98% identity to rsc:RCFBP_11595)

Predicted SEED Role

"Amino acid transporters"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (476 amino acids)

>UW163_RS15035 amino acid permease (Ralstonia solanacearum UW163)
MSLFRTKNVDHMIRAGQSNTGLKKVLGPLDLTFLGVGAIIGTGIFVLTGTGALTAGPALT
LSFIVAALACGFAALCYAEFASTIPVSGSIYTYAYATLGEVVAWIIGWDLMLEYGLATSA
VSVGWSGYFQSLMSGFGVHLPAALTAAPGAVPGVQTLFNLPALLIMLIITAVLSFGVRES
ARFNNVMVAIKVTVVLLFIVVGARHVQPANWHPFMPFGMSGVFGAAALVFFAFIGFDAVT
SAAEEVRNPERDLPIGIIGSLGLCTILYVVVAAIMTGIVPYPKFAGVDHPVSLALQMGGE
TWVAGFVDLGAIIGMTTVILVMGYGQTRVIFAMSRDGLLPKRLSDIHPRYATPFFNTWVV
GIVFALIAAFVPLNVLAELINIGTLAAFSLIAVAVLVLRRKRPELSRGFRCPGVPFVPLA
AVVLCLFLMSQLQALTWIAFVVWMAIGLLVYFGYARSRSLLHVPERGAVPVSETAS