Protein Info for UW163_RS14915 in Ralstonia solanacearum UW163

Annotation: iron-sulfur cluster-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 469 TIGR00273: iron-sulfur cluster-binding protein" amino acids 17 to 451 (435 residues), 456.3 bits, see alignment E=5.2e-141 PF02589: LUD_dom" amino acids 68 to 292 (225 residues), 163.3 bits, see alignment E=2.1e-51 PF13183: Fer4_8" amino acids 306 to 374 (69 residues), 45.8 bits, see alignment E=2.6e-15 PF13534: Fer4_17" amino acids 309 to 374 (66 residues), 28.4 bits, see alignment E=6.6e-10 PF11870: LutB_C" amino acids 392 to 466 (75 residues), 52 bits, see alignment E=2.8e-17

Best Hits

Swiss-Prot: 44% identical to LUTB_BACLD: Lactate utilization protein B (lutB) from Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)

KEGG orthology group: None (inferred from 97% identity to rsc:RCFBP_11619)

Predicted SEED Role

"Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF" in subsystem L-rhamnose utilization or Lactate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (469 amino acids)

>UW163_RS14915 iron-sulfur cluster-binding protein (Ralstonia solanacearum UW163)
MQVHSMEFKARAGQKLADKRLQQNLTKLSTKFVTARAAAIQEIDFAATREALKERRDRAL
ENLDVWLATFEAEATRRGAKVLFAETTADAARLVAEIARRHGVRKVIKSKSMVTEEMRLN
QVLGGMGVQSIETDLGEYILQINDSEPPSHIIAPAVHKDKEEVADLFAKTHHRPRLTDIP
QMTREAREVLRPEFLSADMGVTGGNFIIAETGSVALVTNEGNEGMCTVMPRVHVAVTGIE
KVLPTLEDLATVMRLLPRSATGQAISNYFSLLTGPRAEGEQDGPEHMYFVIVDGGRSGLI
GGEFQEMLRCIRCGACMNHCPVYQKIGGHAYGWVYPGPMGSVLTPSYVGLSNALDLPQAA
TLCGECNRVCPASIPLSDLLRTLREKQMERELRPPSERFGLRLWGFMARRPALYAAGTWL
AARVLRWMGGDTKLIHSLPVGKGWTDTRDMPAPAGKTFRELYREKRVRA