Protein Info for UW163_RS13855 in Ralstonia solanacearum UW163

Annotation: glutamine-hydrolyzing GMP synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00888: GMP synthase (glutamine-hydrolyzing), N-terminal domain
  • amino acids 5 to 199 (195 residues), 228.9 bits, E=3.3e-72
• PF00117: GATase
  • amino acids 6 to 195 (190 residues), 128.3 bits, E=7.5e-41
• PF07722: Peptidase_C26
  • amino acids 68 to 178 (111 residues), 40.8 bits, E=5.5e-14
• TIGR00884: GMP synthase (glutamine-hydrolyzing), C-terminal domain
  • amino acids 208 to 539 (332 residues), 462.7 bits, E=5.9e-143
• PF02540: NAD_synthase
  • amino acids 209 to 249 (41 residues), 29 bits, 1.5e-10
• PF00958: GMP_synt_C
  • amino acids 453 to 538 (86 residues), 136.8 bits, E=5.1e-44

Best Hits

Swiss-Prot: 95% identical to GUAA_RALPJ: GMP synthase [glutamine-hydrolyzing] (guaA) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K01951, GMP synthase (glutamine-hydrolysing) [EC: 6.3.5.2] (inferred from 99% identity to rsc:RCFBP_20002)

Predicted SEED Role

"GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)" in subsystem Purine conversions or Staphylococcal pathogenicity islands SaPI (EC 6.3.5.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• L-citrulline biosynthesis (8/8 steps found)
• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• superpathway of purine nucleotide salvage (12/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• superpathway of L-citrulline metabolism (10/12 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Drug metabolism - other enzymes
• Glutamate metabolism
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.2

Use Curated BLAST to search for 6.3.5.2

Protein Sequence (539 amino acids)

>UW163_RS13855 glutamine-hydrolyzing GMP synthase (Ralstonia solanacearum UW163)
MHDKVLILDFGSQVTQLIARRVREAHVYCEIHPNDVSDAFVREFAPKAVILSGSHASTYE
DHQLRAPQAVWDLGVPVLGICYGMQTMAVQLGGKVEWSDHREFGYAEVRAHGHTRLLKDI
QDFATAEGHGMLKVWMSHGDKVAELPPGFKLLASTPSCPIAGMADESRGYYAVQFHPEVT
HTVQGRALLERFVLEIAGAKPDWIMRDHIEEAVKSIREQVGDEEVILGLSGGVDSSVAAA
LIHRAIGDQLTCVFVDHGLLRLNEGQMVLDMFEGRLHAKVVHANASEQFLGHLAGVTDPE
AKRKIIGREFVEVFQAEAKKLTNAKWLAQGTIYPDVIESGGAKTKKATTIKSHHNVGGLP
ETLGLKLLEPLRDLFKDEVRELGVALGLPPEMVYRHPFPGPGLGVRILGEVKREYADLLR
RADAIFIEELRGTKATAQDVAAGLCAEDQVGKSWYDLTSQAFAVFLPVKSVGVMGDGRTY
DYVVALRAVQTTDFMTAHWAHLPYVLLGRASNRIINEVRGINRVVYDVSGKPPATIEWE