Protein Info for UW163_RS13415 in Ralstonia solanacearum UW163

Annotation: sulfate ABC transporter permease subunit CysT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 306 transmembrane" amino acids 20 to 44 (25 residues), see Phobius details amino acids 72 to 96 (25 residues), see Phobius details amino acids 108 to 130 (23 residues), see Phobius details amino acids 142 to 166 (25 residues), see Phobius details amino acids 195 to 216 (22 residues), see Phobius details amino acids 224 to 243 (20 residues), see Phobius details amino acids 252 to 276 (25 residues), see Phobius details TIGR00969: sulfate ABC transporter, permease protein" amino acids 17 to 278 (262 residues), 334.1 bits, see alignment E=6.7e-104 TIGR02139: sulfate ABC transporter, permease protein CysT" amino acids 19 to 282 (264 residues), 428.5 bits, see alignment E=1.1e-132 PF00528: BPD_transp_1" amino acids 85 to 283 (199 residues), 79 bits, see alignment E=2e-26

Best Hits

Swiss-Prot: 54% identical to CYST_ECOLI: Sulfate transport system permease protein CysT (cysU) from Escherichia coli (strain K12)

KEGG orthology group: K02046, sulfate transport system permease protein (inferred from 97% identity to rsc:RCFBP_20084)

MetaCyc: 54% identical to sulfate/thiosulfate ABC transporter inner membrane subunit CysU (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate transport system permease protein CysT" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (306 amino acids)

>UW163_RS13415 sulfate ABC transporter permease subunit CysT (Ralstonia solanacearum UW163)
MSLSSPFSSAARRRKPANVLPGFGLALGFSLLYLALIVLIPLSATVLKTFSMTWDAFWST
VTAPRVVASYQLTFGASLLAAAINLVFGLTVAWVLVRYRFPGKRLVDALVDLPFALPTAV
AGIALTALYAPNGWIGSRLEPLGLKVAFTPLGIVVALTFIGLPFVVRTVQPVLEDLEAEL
EEAAASLGATRWQTFIRVILPTLLPALLTGFALAFARATGEYGSVIFIAGNMPMISEITP
LMIYSKLEQFDYAGATAIAVVMLAISFALLLVINLLQAWTRRRGSRNDALERAPEPPAGA
NAAAGA