Protein Info for UW163_RS13150 in Ralstonia solanacearum UW163

Annotation: tRNA pseudouridine(55) synthase TruB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 316 TIGR00431: tRNA pseudouridine(55) synthase" amino acids 18 to 229 (212 residues), 260.7 bits, see alignment E=4.8e-82 PF01509: TruB_N" amino acids 39 to 190 (152 residues), 186.5 bits, see alignment E=5.7e-59 PF16198: TruB_C_2" amino acids 191 to 248 (58 residues), 58.7 bits, see alignment E=7.5e-20 PF09157: TruB-C_2" amino acids 252 to 312 (61 residues), 49.7 bits, see alignment E=4.1e-17

Best Hits

Swiss-Prot: 95% identical to TRUB_RALSO: tRNA pseudouridine synthase B (truB) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K03177, tRNA pseudouridine synthase B [EC: 5.4.99.12] (inferred from 96% identity to rsc:RCFBP_20136)

Predicted SEED Role

"tRNA pseudouridine synthase B (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (316 amino acids)

>UW163_RS13150 tRNA pseudouridine(55) synthase TruB (Ralstonia solanacearum UW163)
MAEQRPSQPAKPPRHDVHGVLLLDKPIGWSSNDALIRAKRLLWAKKAGHTGTLDPLATGL
LPLCFGEATKFSQDLLEADKTYETVVRLGIKTSTADAEGEVLSERPVAVTPEQLRAAVDR
FIGEIDQVPPMHSALKKDGKPLYEYARAGQTVERAARRVTIRAIDVLATDLDAAAPTVTL
RVCCSKGTYIRTLGEDLGEALGCGAHLVALRRTQVGSLTLDGAVTLQALEAASEDQRAAL
LAPVDALLQTLPRVELGAEDSRRFLHGQRLPLRLSLPGAEQVRVYGARGEAALSLLGVAA
WQDGVLRPERLVHSTR