Protein Info for UW163_RS12770 in Ralstonia solanacearum UW163

Annotation: GTPase HflX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03156: GTP-binding protein HflX
  • amino acids 13 to 366 (354 residues), 470.2 bits, E=1.8e-145
• PF13167: GTP-bdg_N
  • amino acids 28 to 114 (87 residues), 103.6 bits, E=2.5e-33
• PF16360: GTP-bdg_M
  • amino acids 117 to 196 (80 residues), 89.1 bits, E=7.8e-29
• PF02421: FeoB_N
  • amino acids 203 to 360 (158 residues), 38 bits, E=4.3e-13
• PF01926: MMR_HSR1
  • amino acids 204 to 322 (119 residues), 82.1 bits, E=1.2e-26

Best Hits

Swiss-Prot: 55% identical to HFLX_ECOLI: GTPase HflX (hflX) from Escherichia coli (strain K12)

KEGG orthology group: K03665, GTP-binding protein HflX (inferred from 98% identity to rsc:RCFBP_20212)

MetaCyc: 55% identical to ribosome rescue factor HflX (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein HflX" in subsystem Hfl operon or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (417 amino acids)

>UW163_RS12770 GTPase HflX (Ralstonia solanacearum UW163)
MPSHPTSSTEPTRAILVAVDFGKHDFQESLSELALLASTAGSEPVRAVTGRRSRPDAALF
IGAGKAEEVKIAADEDDAEIVIFNHALSPAQQRNLERFFGRHVVDRTGLILDIFSQRAQS
HVGKVQVELARVRYQASRLVRAWSHLERQKGGIGMRGGPGERQLELDRRMLDERAKRLSA
ELDRLQRQHDTQRRSRSRNDAFSVSLVGYTNAGKSTLFNALTKARAYAANQLFATLDTTS
RRLYLEGLGNVVLSDTVGFIRDLPTQLVAAFRATLEETVHADVLLHVVDAASAVKHEQME
QVDRVLAEIDASGIPQILVMNKIDAAEELRAQGPRIERDETGAVRRVFVSALEGAGLELL
REALVETAIRLRERPPPRDGEFDPRFDTRQETSPSQRDELSQIPPASALPAERSGRD