Protein Info for UW163_RS12105 in Ralstonia solanacearum UW163

Annotation: salicylate 5-hydroxylase large oxygenase NagG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00355: Rieske
  • amino acids 44 to 129 (86 residues), 63.5 bits, E=1.4e-21
• PF00848: Ring_hydroxyl_A
  • amino acids 202 to 417 (216 residues), 60.7 bits, E=1.9e-20

Best Hits

Swiss-Prot: 79% identical to NAGG_RALSP: Salicylate 5-hydroxylase, large oxygenase component (nagG) from Ralstonia sp.

KEGG orthology group: K05708, large terminal subunit of phenylpropionate dioxygenase [EC: 1.14.12.19] (inferred from 96% identity to rso:RSc1090)

MetaCyc: 79% identical to salicylate-5-hydroxylase large subunit (Ralstonia sp. U2)
RXN-10446 [EC: 1.14.13.172]

Predicted SEED Role

"Ortho-halobenzoate 1,2-dioxygenase alpha-ISP protein OhbB" in subsystem Benzoate degradation

MetaCyc Pathways

• salicylate degradation II (1/1 steps found)
• salicylate glucosides biosynthesis II (1/2 steps found)
• 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-hydroxypentadienoate (2/5 steps found)
• cinnamate and 3-hydroxycinnamate degradation to 2-hydroxypentadienoate (2/5 steps found)
• 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation (2/8 steps found)

KEGG Metabolic Maps

• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.19

Use Curated BLAST to search for 1.14.12.19 or 1.14.13.172

Protein Sequence (418 amino acids)

>UW163_RS12105 salicylate 5-hydroxylase large oxygenase NagG (Ralstonia solanacearum UW163)
MKASPLTFSTPLWKDSGSSRIPFRAYTDEDIYRRELERLFYAGHWCYVGLEAEIPNPGDF
KRTAIGERSVIVTRTPDHGIAVVENVCAHRGVRFCREKSGNRKDFTCPYHQWNYDLKGNL
IGVPFRRGVKADGKVNGGMPADFDPKQHGLTQLNVAVRNGVIFASFDHGLPSLETFLGPT
ILGYFDRVFDGRKLKILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNKS
ELRMDALHRHAAMISTRGSGGKGAVTSGVSSFKEAMTLHDPRFLDVVHEPWWGTPTAVMM
TIFPSVIVQQQVNSVSTRHIQPNGHGAFDFVWTHFGFEDDTEEMTQRRLRQANLFGPAGF
VSADDGEVIEFSQQGFEQKPGHQTIAELGGREVGETDHMVTETLIRGMYEYWRTVMEL