Protein Info for UW163_RS10815 in Ralstonia solanacearum UW163

Annotation: NADH-quinone oxidoreductase subunit NuoN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01770: proton-translocating NADH-quinone oxidoreductase, chain N
  • amino acids 6 to 475 (470 residues), 452.2 bits, E=1.2e-139
• PF00361: Proton_antipo_M
  • amino acids 125 to 424 (300 residues), 245.7 bits, E=3.1e-77

Best Hits

Swiss-Prot: 72% identical to NUON_CUPTR: NADH-quinone oxidoreductase subunit N (nuoN) from Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1)

KEGG orthology group: K00343, NADH dehydrogenase I subunit N [EC: 1.6.5.3] (inferred from 99% identity to rsc:RCFBP_11339)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• NADH to cytochrome bd oxidase electron transfer I (1/2 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)
• Fe(II) oxidation (2/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Protein Sequence (490 amino acids)

>UW163_RS10815 NADH-quinone oxidoreductase subunit NuoN (Ralstonia solanacearum UW163)
MQNMNWAPLLPIIFLSVMIAVIQLADVYRREQSNSPAYCLSLFTTLALTVWFAVQAASGE
THYVFSNMAVFDPMAQVLSSFCALATLVTFVYTRTYLADRDMFSGEFYILALFTLLGQVV
MISGNNFLTLYLGLELLSLGSYALVALRRRSNVSSESGMKYFILGALASGLLLYGMSMLY
GATGSLDLGKVFHAIESGDINKTILVFGVVFIVAGLAFKLGAAPFHMWVPDVYQGSPTAV
TLLIAGAPKVAAFAMTLRILVEGLLPLAFDWQNMLIVLAVASLAIGNITGVVQTNFKRLL
AYSTVSHMGFLLLGLLSGVSSGKADLTAQAYSASMFYAITYVLTALGAFGIILLLARKGY
EAEEIADLRGLSKKSPWFALLTLFMMMSLAGLPPTVGFYAKLSVLSAVIDAGMPWLAIVA
VLFSLVGAFYYLRVVKAMYFDAPADDVPVQATFGLRSLLSVNGLLVLVLGLFPAGLMGLC
YQAIRLTLAS