Protein Info for UW163_RS10775 in Ralstonia solanacearum UW163

Annotation: NADH-quinone oxidoreductase subunit F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01959: NADH oxidoreductase (quinone), F subunit
  • amino acids 20 to 420 (401 residues), 640.1 bits, E=5.2e-197
• PF01512: Complex1_51K
  • amino acids 53 to 224 (172 residues), 156.7 bits, E=6.8e-50
• PF10531: SLBB
  • amino acids 248 to 296 (49 residues), 44.7 bits, 1.5e-15
• PF10589: NADH_4Fe-4S
  • amino acids 338 to 419 (82 residues), 107.6 bits, E=3.6e-35

Best Hits

Swiss-Prot: 51% identical to NUOF2_RHIME: NADH-quinone oxidoreductase subunit F 2 (nuoF2) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00335, NADH dehydrogenase I subunit F [EC: 1.6.5.3] (inferred from 99% identity to rso:RSc2057)

MetaCyc: 49% identical to NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (Homo sapiens)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• NADH to cytochrome bd oxidase electron transfer I (1/2 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)
• Fe(II) oxidation (2/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Protein Sequence (431 amino acids)

>UW163_RS10775 NADH-quinone oxidoreductase subunit F (Ralstonia solanacearum UW163)
MTSLHDRHIQPLILAGLNGDNWHLEDYVKRGGYQQLKRILTEKISPEQVIADVKASGLRG
RGGAGFPTGLKWSFMPRQFPGQKYLVCNTDEGEPGTFKDRDIIRYNPHALIEGMAIGGYA
MGITVGYNYIHGEIWNEYKIFEQALEEARAAGFLGDNILGSGFNFQLHAHHGYGAYICGE
ETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFAAVPFLLAIGPDNYLKMGKP
NNGGSKIFSVSGDVERPGNYEIPLGTPFSKLLELAGGMRGGKKLKAVIPGGSSAPVVPAD
LMMASDMDYDSIAKAGSMLGSGAVIVMDETRCMVKSLLRLSYFYFEESCGQCTPCREGTG
WLYRVVDRIEHGKGRQEDLDLLNNVAENIMGRTICALGDAAAMPVRGMLKHYWDEFAYHV
EHKQCMVPTYI