Protein Info for UW163_RS10160 in Ralstonia solanacearum UW163

Annotation: quinone oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 324 PF08240: ADH_N" amino acids 28 to 86 (59 residues), 36.1 bits, see alignment E=7.3e-13 PF00107: ADH_zinc_N" amino acids 151 to 255 (105 residues), 91.6 bits, see alignment E=6.2e-30 PF13602: ADH_zinc_N_2" amino acids 184 to 322 (139 residues), 78.2 bits, see alignment E=1.9e-25

Best Hits

Swiss-Prot: 59% identical to QOR_PSEAE: Quinone oxidoreductase (qor) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00344, NADPH2:quinone reductase [EC: 1.6.5.5] (inferred from 99% identity to rsc:RCFBP_11212)

MetaCyc: 48% identical to 2-haloacrylate reductase (Burkholderia sp. WS)
RXN-14536 [EC: 1.3.1.103]

Predicted SEED Role

"Quinone oxidoreductase (EC 1.6.5.5)" in subsystem ZZ gjo need homes (EC 1.6.5.5)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.5

Use Curated BLAST to search for 1.3.1.103 or 1.6.5.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (324 amino acids)

>UW163_RS10160 quinone oxidoreductase (Ralstonia solanacearum UW163)
MTKAIRIVETGGPEVMQLVDVDVPAPGPGEARVRQHAIGLNYIDVYFRSGLYPQPLPGGI
GMEAAGVVEAVGEGVAHVKPGDRVAYAGRPTGAYAEVRTMPADILVRLPDAIDFETGAAM
MLQGLTTQYLLRDSYRVQPGDTVLFHAAAGGVGLIACQWLKALGATVIGTVGSDAKAELA
RAHGCDHTIVYTRENFTERVREITGGKGVPVVYDGIGKDTFAGSLDCLALRGMMVSYGNA
SGPVPPFDLGVLSAKGSLKLTRPTLMTYTAQRALLEPMAAELFDVVASGKVKIEIHQRYA
LADAAQAHRDLESRKTTGSTILLP