Protein Info for UW163_RS09950 in Ralstonia solanacearum UW163

Annotation: aromatic ring-hydroxylating dioxygenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 365 PF00355: Rieske" amino acids 43 to 123 (81 residues), 45.4 bits, see alignment E=6.2e-16 PF00848: Ring_hydroxyl_A" amino acids 170 to 364 (195 residues), 139.8 bits, see alignment E=1.2e-44

Best Hits

KEGG orthology group: None (inferred from 99% identity to rsc:RCFBP_11169)

MetaCyc: 63% identical to putrescine 2-hydroxylase (Bordetella bronchiseptica)
1.14.13.M64 [EC: 1.14.13.M64]

Predicted SEED Role

"Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit" in subsystem Phenylpropanoid compound degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.13.M64

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (365 amino acids)

>UW163_RS09950 aromatic ring-hydroxylating dioxygenase subunit alpha (Ralstonia solanacearum UW163)
MSNLSTALNLVPSETQLPVSAYFDEALYQTEIERLFKHGPGYVGHELMVPEVGDYHTLAA
EAEGRVLVRNPNGVELLSNVCRHRQAIMLNGRGNAQNIVCPLHRWTYDLKGELLGAPHFE
QQPCVHLSRSLLQNWNGLLFEGKRDVRNDLARLGVTRDLDFSGYILDHVEVHDCNYNWKT
FIEVYLEDYHVVPFHPGLGQFVSCDDLTWEFGEWYSVQTVGIHAGLRRPGTPTYQKWHDA
VLRFNNGEMPKYGAVWLTYYPNVMVEWYPNVLVVSTLHPMGPTRTRNVVEFYYPEEIALF
EREFVEAERAAYMETCIEDDEIAERMDAGRLALLKRGTSEVGPYQSPMEDGMQHFHEWYR
RVMDY