Protein Info for UW163_RS09820 in Ralstonia solanacearum UW163

Annotation: 3-oxoadipate enol-lactonase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02427: 3-oxoadipate enol-lactonase
  • amino acids 10 to 260 (251 residues), 347.5 bits, E=2e-108
• PF12146: Hydrolase_4
  • amino acids 21 to 231 (211 residues), 40.7 bits, E=3.6e-14
• PF00561: Abhydrolase_1
  • amino acids 24 to 133 (110 residues), 63.4 bits, E=5.2e-21
• PF12697: Abhydrolase_6
  • amino acids 27 to 253 (227 residues), 42 bits, E=3.6e-14

Best Hits

Swiss-Prot: 44% identical to ELH2_ACIAD: 3-oxoadipate enol-lactonase 2 (catD) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K01055, 3-oxoadipate enol-lactonase [EC: 3.1.1.24] (inferred from 97% identity to rsc:RCFBP_11145)

MetaCyc: 50% identical to subunit of 3-oxoadipate enol-lactone hydrolase (Pseudomonas putida)
3-oxoadipate enol-lactonase. [EC: 3.1.1.24]

Predicted SEED Role

"Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24)" in subsystem Catechol branch of beta-ketoadipate pathway or Chloroaromatic degradation pathway or Protocatechuate branch of beta-ketoadipate pathway (EC 3.1.1.24)

MetaCyc Pathways

• aromatic compounds degradation via β-ketoadipate (9/9 steps found)
• superpathway of salicylate degradation (7/7 steps found)
• catechol degradation III (ortho-cleavage pathway) (6/6 steps found)
• catechol degradation to β-ketoadipate (4/4 steps found)
• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• 4-methylcatechol degradation (ortho cleavage) (4/7 steps found)
• mandelate degradation to acetyl-CoA (9/18 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (17/35 steps found)
• superpathway of aerobic toluene degradation (10/30 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.24

Use Curated BLAST to search for 3.1.1.24

Protein Sequence (270 amino acids)

>UW163_RS09820 3-oxoadipate enol-lactonase (Ralstonia solanacearum UW163)
MPWLEHDNVRLYHEFDDRHASAKPVLVLSNSLGTHLGMWAPQLEALRRHFRLVRYDTRGH
GQSSVPDAPFGVAQLGGDVLALLDHYDIELALFCGLSMGGLTGLWLAAHHGERFPRMVVS
NTAALIGTPQSWNARIAQVEQGGMAGITGTVLERWFTDGYRVAAPERVDLVKAMLLATPP
AGYNGNCAAIRDADLRAQLQDIRVPLLAIGGTHDISTPAAQTRAIAQGVPGAQYVELSAG
HLANWEQAEAYTDALIGFLTTGVAREAAHG