Protein Info for UW163_RS09815 in Ralstonia solanacearum UW163

Annotation: 3-carboxy-cis,cis-muconate cycloisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02426: 3-carboxy-cis,cis-muconate cycloisomerase
  • amino acids 5 to 348 (344 residues), 475.9 bits, E=3e-147
• PF00206: Lyase_1
  • amino acids 12 to 300 (289 residues), 174.1 bits, E=4.9e-55
• PF10397: ADSL_C
  • amino acids 364 to 444 (81 residues), 64.6 bits, E=8.4e-22

Best Hits

Swiss-Prot: 56% identical to PCAB_PSEAE: 3-carboxy-cis,cis-muconate cycloisomerase (pcaB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 98% identity to rsc:RCFBP_11144)

Predicted SEED Role

"3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 5.5.1.2)

MetaCyc Pathways

• aromatic compounds degradation via β-ketoadipate (9/9 steps found)
• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (17/35 steps found)
• superpathway of aerobic toluene degradation (10/30 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.5.1.2

Protein Sequence (453 amino acids)

>UW163_RS09815 3-carboxy-cis,cis-muconate cycloisomerase (Ralstonia solanacearum UW163)
MTTGLLDRAFSTPAMLAVWSDAATVRAMLDVEAALAQAEGEAGVIPAQAVAPIRAVCATA
TPDLDALGTAAAGAGNLAIPLVKQLTAAVARYDEHAARYVHWGATSQDIIDTGLMLQWRQ
AAALLDADLRQLADALAALARTHRATPMVARTWLQQALPTTFGRKAAGWLAAVHRAQDRF
AALRAHVPALQFGGAAGTLASLGAHGRAVAQALAQALDLPLPAASWHGEQDRMADIGAAL
ALLTGTLGHLARDLSLMMQTEVGEVAEPAGAGKGGSSTMPHKRNPVGCATVLAAATRMPG
LAATLLAALPQEHERALGGWQAQWATLREMACLAAGALDRMRDLIAGLDVDPARMRANLD
LTHGLILGEAVMLALGADLGRLQAHHVVEAASRRAVEQGRTLRDVLAEDADVVRTWGDAA
SQRLDALLDPAGYLGDAAAAVDRVLAEHDRRRA