Protein Info for UW163_RS09320 in Ralstonia solanacearum UW163

Annotation: formate dehydrogenase subunit gamma

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01583: formate dehydrogenase, gamma subunit
  • amino acids 155 to 359 (205 residues), 163 bits, E=4.7e-52
• PF01292: Ni_hydr_CYTB
  • amino acids 158 to 338 (181 residues), 68.4 bits, E=3.5e-23

Best Hits

KEGG orthology group: K00127, formate dehydrogenase, gamma subunit [EC: 1.2.1.2] (inferred from 96% identity to rsc:RCFBP_11051)

Predicted SEED Role

"Formate dehydrogenase O gamma subunit (EC 1.2.1.2)" in subsystem Formate dehydrogenase or Formate hydrogenase (EC 1.2.1.2)

MetaCyc Pathways

• formate oxidation to CO₂ (1/1 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• oxalate degradation VI (1/4 steps found)
• oxalate degradation III (1/5 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Protein Sequence (387 amino acids)

>UW163_RS09320 formate dehydrogenase subunit gamma (Ralstonia solanacearum UW163)
MIACWKPLATALLLGGAVTLASAQQPASTPAAAPVNPAVQPAQPFSNVVSENVFNLPRRD
LAKEAQDQTARRVTQPGNSAPFWREVNSDQANYASLPGLETSVLIQRTGQKWRLFRNGVI
TVWGGWLVVLVPLAILGVFLLKGQIRLKEAPTGRKIERFTPTERYVHWTMAISFVTLGVS
GIAMLWGKHFLLPLMGHQLFGWLTYVLKNLHNLVGPLFTVSIVVAFAMWARDNLPRRGDL
QWLLRLGGMFGGHGAEVPSHRFNAGEKLWFWGGLVVLGLILSASGWVLDRIVPGVDYYRS
TMQLAEIIHAIAAVGMICMSLGHIYIGTIGMEGAYRAMRDGYVDEAWAREHHVLWYDDIQ
AGKIPAQRSRPPDPPELPEPREQPAKG