Protein Info for UW163_RS08685 in Ralstonia solanacearum UW163

Annotation: DNA-3-methyladenine glycosylase 2 family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 493 PF02805: Ada_Zn_binding" amino acids 7 to 71 (65 residues), 103.5 bits, see alignment E=9.5e-34 PF12833: HTH_18" amino acids 106 to 185 (80 residues), 76.7 bits, see alignment E=2.9e-25 PF06029: AlkA_N" amino acids 197 to 312 (116 residues), 128.5 bits, see alignment E=3.1e-41

Best Hits

KEGG orthology group: K13529, AraC family transcriptional regulator, regulatory protein of adaptative response / DNA-3-methyladenine glycosylase II [EC: 3.2.2.21] (inferred from 97% identity to rsc:RCFBP_10893)

Predicted SEED Role

"ADA regulatory protein" in subsystem DNA repair, bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.21

Use Curated BLAST to search for 3.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (493 amino acids)

>UW163_RS08685 DNA-3-methyladenine glycosylase 2 family protein (Ralstonia solanacearum UW163)
MHLDHDACYHAVQSRDRRFDGWFFVGVTSTGVYCRPICAVRTPLQKNCRFFATAAAAEKA
GFRPCLRCRPELAPGHSLAEMSSSLARAAARMIDEGFLQQHDLAALAAAVGVTDRHLRRI
FRTEFDVSPIEYAQTQRLLLAKQLLTDTDMPVGDIAYAAGFGSVRRLNSGFAEHYGFAPT
RLRTRAAPVPRSDDGATLMLGYRPPFAWHALLDFLRARAVDGVETVDAHSYARTIALDYA
GRRHAGWLHARDVPQRHAVALTLSASLMQAMPPVLARVRRLFDLDCRPDLVDAHLGTLAA
GTPGLRVPGAVDGFEIAVRAIAGQVVSLAQARRILARLVVAHGTPLTQPRAGLPMAFPSA
TALAGSDPLTLSRETGLQASRAAAVVEIARAVDAGRLRLEPLVPLVPLAPTLAALQALPG
IGEWTAQYIAMRALGWPNAFPLGDYVLRKRLADHDDALPTRHAMATRAEGWAPWRAYAAM
HLWHHDVALAPTP