Protein Info for UW163_RS08130 in Ralstonia solanacearum UW163
Annotation: glycolate oxidase subunit GlcE
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K11472, glycolate oxidase FAD binding subunit (inferred from 94% identity to rsc:RCFBP_10781)Predicted SEED Role
"Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.99.14)
MetaCyc Pathways
- glycolate and glyoxylate degradation II (2/2 steps found)
- glycolate and glyoxylate degradation I (3/4 steps found)
- superpathway of glycol metabolism and degradation (5/7 steps found)
- glycolate and glyoxylate degradation III (1/3 steps found)
- photorespiration III (5/9 steps found)
- photorespiration II (5/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.99.14
Use Curated BLAST to search for 1.1.99.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (381 amino acids)
>UW163_RS08130 glycolate oxidase subunit GlcE (Ralstonia solanacearum UW163) MTAPDPALARFRDGILQAAASRAPLTLRGGGTKDFYGRAPAPGATVLDTRAWSGIVDYDP AELVVTARSGTPLDEVEAALAEHRQMLAFEPPHFAGGGRQATVGGAFAAGLSGPRRQSVG ALRDFVLGAVVMDGRGDLLNFGGQVMKNVAGYDVSRLMAGALGTLGLVVQVSLKVLPMPF GDATLRFAMPQAEAIDTLNRWGGQPLPIAASAWIGDTLWVRLCGAEAAVRAARAKLGGDR IDEAEALALWRDLREQSHAFFARAVQGGLPLWRIALPPATPPLALGHAAADEQLIEWGGG QRWWIDTSGTGTIGTMGNTPAEAIRDFALRAGGHATLFRNGDRDGAVFTPVAAPLMAVHK RLKDSFDPHGIFNPGRLYADL