Protein Info for UW163_RS07995 in Ralstonia solanacearum UW163

Annotation: Zn-dependent hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 TIGR01879: amidase, hydantoinase/carbamoylase family" amino acids 17 to 415 (399 residues), 402.1 bits, see alignment E=1.3e-124 PF04389: Peptidase_M28" amino acids 71 to 146 (76 residues), 39.1 bits, see alignment E=1e-13 PF01546: Peptidase_M20" amino acids 89 to 415 (327 residues), 101.3 bits, see alignment E=1.1e-32 PF07687: M20_dimer" amino acids 221 to 321 (101 residues), 32.8 bits, see alignment E=8.6e-12

Best Hits

Swiss-Prot: 41% identical to HYUC_RHIML: N-carbamoyl-L-amino-acid hydrolase (hyuC) from Rhizobium meliloti

KEGG orthology group: None (inferred from 96% identity to rsc:RCFBP_10747)

MetaCyc: 41% identical to N-carbamoyl-L-amino-acid hydrolase subunit (Sinorhizobium meliloti CECT4114)
N-carbamoyl-L-amino-acid hydrolase. [EC: 3.5.1.87]

Predicted SEED Role

"Beta-ureidopropionase (EC 3.5.1.6)" in subsystem Hydantoin metabolism or L-2-amino-thiazoline-4-carboxylic acid-Lcysteine conversion or Pyrimidine utilization (EC 3.5.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.6 or 3.5.1.87

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (428 amino acids)

>UW163_RS07995 Zn-dependent hydrolase (Ralstonia solanacearum UW163)
MQAHQVTHPELELDGARLLAQLRALGEVGADPDAGGRTRIALTDDERAGRDLVVRWMRAL
DLDVRIDRIGNIFGTLRSGSDTGSEAPLMIGSHIDTVTHAGALDGCYGVLAGLAVAHAFR
QAGIRPHRSITIAAFTNEEGVRYQPDMMGSLVYAGGLALEDALNTIGTDGTKLGEELARI
GYAGDMEPGAVVPHAYLELHIEQGPILEADNTLIGVVQNLQGISWQQVTVQGNANHAGTT
PTHLRHDAGWTACAIVDFLRELAVASGGTTLATVGCMRFEPNVINVIPRRATFTVDLRDP
DEARLQAAEKRLADFLNAIAEREGVKIGTEQLVRFEPVVFDREIADEIEASAKRLGLSHR
RMTSGAGHDAQMIARIAPSAMIFVPSRGGISHNPREHTDDDQLVMGARVLLDVVLRRLGQ
SDIRAAGR