Protein Info for UW163_RS07170 in Ralstonia solanacearum UW163

Annotation: phenylacetate--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02155: phenylacetate-CoA ligase
  • amino acids 14 to 432 (419 residues), 685.1 bits, E=1.4e-210
• PF00501: AMP-binding
  • amino acids 85 to 291 (207 residues), 62.7 bits, E=2.9e-21
• PF14535: AMP-binding_C_2
  • amino acids 339 to 432 (94 residues), 83.7 bits, E=9e-28

Best Hits

Swiss-Prot: 73% identical to PAAK_AZOEV: Phenylacetate-coenzyme A ligase (paaK) from Azoarcus evansii

KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 99% identity to rsc:RCFBP_10583)

MetaCyc: 73% identical to phenylacetate-CoA ligase (aerobic) (Aromatoleum evansii)
Phenylacetate--CoA ligase. [EC: 6.2.1.30]

Predicted SEED Role

"Phenylacetate-coenzyme A ligase (EC 6.2.1.30)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 6.2.1.30)

MetaCyc Pathways

• superpathway of phenylethylamine degradation (9/11 steps found)
• phenylacetate degradation I (aerobic) (7/9 steps found)
• L-phenylalanine degradation IV (mammalian, via side chain) (5/9 steps found)
• penicillin G and penicillin V biosynthesis (1/4 steps found)
• phenylacetate degradation II (anaerobic) (1/4 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (6/27 steps found)

KEGG Metabolic Maps

• Phenylalanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.30

Protein Sequence (436 amino acids)

>UW163_RS07170 phenylacetate--CoA ligase (Ralstonia solanacearum UW163)
MRPRSTDLPLDPIETASRDAVQALQLERLRHSLVHAYANVPAYRAKFDAAGVHPSDLRSL
DDLAKFPFTTKADLRDNYPFGMFAVPQERIARIHASSGTTGKPTVVGYTLADIDTWAGLV
ARSIRAAGARRGDKVHVSYGYGLFTGGLGAHYGAERAGLTVIPFGGGQTERQVQLIHDFR
PQIIMVTPSYMLAIADEFARQGLDPAASSLQIGILGAEPWTPEMRTAIEARMGLSAVDIY
GLSEVMGPGVASECAETKDGPTLWEDHFYPEIIDPTTGAVLPDGAFGELVFTSLTKEAMP
VVRYRTRDLTRLLPGTARPAFRRMEKITGRCDDMMIVRGVNVFPSQIEELILKHAALAPH
YQCVLAKEGPLDTLTVRVEAALGIPAEAADAARGQLARDIKAMIGVTAAIELLAAGGIER
SVGKARRVVDLRRGQA