Protein Info for UW163_RS05030 in Ralstonia solanacearum UW163

Annotation: hydroxypyruvate isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 260 TIGR03234: hydroxypyruvate isomerase" amino acids 2 to 253 (252 residues), 373 bits, see alignment E=3.4e-116 PF01261: AP_endonuc_2" amino acids 21 to 253 (233 residues), 166 bits, see alignment E=6.8e-53

Best Hits

Swiss-Prot: 60% identical to HYI_ECOLI: Hydroxypyruvate isomerase (hyi) from Escherichia coli (strain K12)

KEGG orthology group: K01816, hydroxypyruvate isomerase [EC: 5.3.1.22] (inferred from 97% identity to rsc:RCFBP_10197)

MetaCyc: 60% identical to hydroxypyruvate isomerase (Escherichia coli K-12 substr. MG1655)
Hydroxypyruvate isomerase. [EC: 5.3.1.22]

Predicted SEED Role

"Hydroxypyruvate isomerase (EC 5.3.1.22)" (EC 5.3.1.22)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.22

Use Curated BLAST to search for 5.3.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (260 amino acids)

>UW163_RS05030 hydroxypyruvate isomerase (Ralstonia solanacearum UW163)
MPKLAANLTMLFNEHAFLERFEAAARAGFRGVEFLFPYAFHADQIADRLSRFQLDLVLHN
LPAGHWDKGERGIAVLPDRVGEFQDGVGEAIRYARALGVRQLNCLVGIRPASVSEDSARR
TLVGNLRFAAGALAAEKIDLLIEPINTFDIPGFYLSRTQQALDLIADVGADNLFVQYDIY
HMQRMEGEVANTLRRHLDTIRHVQLADNPGRNEPGTGEINYPFLFRWLDEIGYGGWVGCE
YKPKTGTVEGLGWRAVHKVA