Protein Info for UW163_RS04950 in Ralstonia solanacearum UW163

Annotation: glycine dehydrogenase (aminomethyl-transferring)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 982 transmembrane" amino acids 260 to 275 (16 residues), see Phobius details amino acids 352 to 372 (21 residues), see Phobius details amino acids 787 to 809 (23 residues), see Phobius details PF02347: GDC-P" amino acids 32 to 462 (431 residues), 580.5 bits, see alignment E=4.4e-178 amino acids 480 to 759 (280 residues), 43.7 bits, see alignment E=3.5e-15 TIGR00461: glycine dehydrogenase" amino acids 33 to 973 (941 residues), 1467.1 bits, see alignment E=0 PF21478: GcvP2_C" amino acids 801 to 922 (122 residues), 196 bits, see alignment E=3.3e-62

Best Hits

Swiss-Prot: 68% identical to GCSP1_PSEAE: Glycine dehydrogenase (decarboxylating) 1 (gcvP1) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00281, glycine dehydrogenase [EC: 1.4.4.2] (inferred from 65% identity to apt:APA01_24220)

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (982 amino acids)

>UW163_RS04950 glycine dehydrogenase (aminomethyl-transferring) (Ralstonia solanacearum UW163)
MNAPHPASSALANTLAERPTLAALEARDAFAERHIGPSSDEQATMLATLGYASRAALIDA
VIPPAIRRRDGMPLGEFTQPLTEEAALAKLRGIAGQNRVAKSLIGQGYYGTHTPGVILRN
ILENPAWYTAYTPYQPEISQGRLEAMLNFQQMVMDLTAMDIANASMLDEATAAAEAMTLL
QRVGKHQSNVFFVADDVLPQTLEVVRTRAKPIGVEVVSGPAADAAKHNAFGVLLQYPGAN
GALLGDLNTYKALTDAVHAAGGLVVAAADLLALTLLAAPGEWGADVAIGNTQRFGVPFGF
GGPHAGYMAVRDAFKRSMPGRLVGVTVDAQGNKAYRLALQTREQHIRREKATSNICTAQV
LLGVMASMYAVYHGPQGLKRIAQRVHRLTATLAAGLRTIGYTLEADAFFDTLTVATGPRT
ANLHVAAQAHGVNLRQIDNARLGISLDETVTRADVVALWDVFAHAAHAATPDFDKVEASV
ADAFPASLLRQSTYLTHPVFNTHHSEHEMLRYLRSLADKDLALDRTMIPLGSCTMKLNAT
AEMLPVTWPEFANIHPFAPADQTVGYREMIDQLEQMLCAATGYAAVSLQPNAGSQGEYAG
LLIIHAYHASRGEGHRDVCLIPSSAHGTNPASAQMAGMKVVVVACDERGNVDLADLEKKA
AEHSANLAAIMITYPSTHGVFEEGVKRVCEIVHSHGGQVYVDGANMNAMVGTAAPGHFGG
DVSHLNLHKTFCIPHGGGGPGVGPVAVGAHLAPFLPGRAASGEDASRNIGAVSAAPFGSA
SILPISWMYIAMMGAAGLTAATETAILSANYVAKRLSPYYPVLYTGAHDLVAHECILDIR
PLQKESGISNEDIAKRLMDFGFHAPTMSFPVPGTLMIEPTESEPKVELDRFIDAMIAIRG
EVDKVISGAFDREDNPLKHAPHTAQVVMADDWSHQYTREQAAYPVASLRARKYWPPVGRA
DNVYGDRNLFCACVPMSEYAQD