Protein Info for UW163_RS04495 in Ralstonia solanacearum UW163

Annotation: 5'-methylthioadenosine/adenosylhomocysteine nucleosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01048: PNP_UDP_1
  • amino acids 11 to 250 (240 residues), 172.2 bits, E=6.3e-55
• TIGR01704: MTA/SAH nucleosidase
  • amino acids 12 to 246 (235 residues), 130.8 bits, E=2.7e-42

Best Hits

KEGG orthology group: K01243, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase [EC: 3.2.2.9] (inferred from 98% identity to rsc:RCFBP_10087)

Predicted SEED Role

"5'-methylthioadenosine nucleosidase (EC 3.2.2.16) @ S-adenosylhomocysteine nucleosidase (EC 3.2.2.9)" (EC 3.2.2.16, EC 3.2.2.9)

MetaCyc Pathways

• S-methyl-5'-thioadenosine degradation IV (1/1 steps found)
• S-adenosyl-L-methionine salvage I (3/4 steps found)
• autoinducer AI-2 biosynthesis II (Vibrio) (4/6 steps found)
• S-methyl-5'-thioadenosine degradation I (1/2 steps found)
• autoinducer AI-2 biosynthesis I (3/5 steps found)
• 5'-deoxyadenosine degradation II (1/4 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (3/7 steps found)
• L-methionine salvage cycle I (bacteria and plants) (4/12 steps found)
• L-methionine salvage cycle II (plants) (2/11 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.2.16 or 3.2.2.9

Protein Sequence (261 amino acids)

>UW163_RS04495 5'-methylthioadenosine/adenosylhomocysteine nucleosidase (Ralstonia solanacearum UW163)
MNIQSPSPNGPIGIVAALHEEISELLTLLSDARRVRIGSRDFWQGELDGRAVVVVLSRIG
KVAAATTAAVLVTQFGVRAVVFAGVAGALASQVAVGDVVVSTELLQHDVDASPLFPRWEI
PLTGVARFQADPALVQGLLESARATLGDPCALLGRRVMEAFGIAAPQAHTGLVISGDRFV
ATSAESRMLRDALPDALAVEMEGAAVAQVCHEWGVPFAAIRTISDRADDAASVDFSQFIA
EVDSRYSVAILRRWLSGTHSA