Protein Info for UW163_RS04200 in Ralstonia solanacearum UW163

Annotation: DNA polymerase III subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 371 PF00712: DNA_pol3_beta" amino acids 6 to 119 (114 residues), 98.4 bits, see alignment E=4.8e-32 TIGR00663: DNA polymerase III, beta subunit" amino acids 6 to 370 (365 residues), 329.7 bits, see alignment E=1.1e-102 PF02767: DNA_pol3_beta_2" amino acids 130 to 248 (119 residues), 120.5 bits, see alignment E=7.3e-39 PF02768: DNA_pol3_beta_3" amino acids 251 to 370 (120 residues), 118.1 bits, see alignment E=3.2e-38

Best Hits

Swiss-Prot: 37% identical to DPO3B_CAUVC: Beta sliding clamp (dnaN) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K02338, DNA polymerase III subunit beta [EC: 2.7.7.7] (inferred from 100% identity to rsl:RPSI07_0002)

Predicted SEED Role

"DNA polymerase III beta subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (371 amino acids)

>UW163_RS04200 DNA polymerase III subunit beta (Ralstonia solanacearum UW163)
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKNGERVSFLSTDIEIQITTHADC
GAGNGDIATTVAARKLVDILRAMPDGEVTLTLNDKRMSVQSGKSRFALQTLAAEEFPTVA
EANDFGARITLPQKTLKHLLAMVHFAMAQQDIRYYLNGMLLVVDGKQVMAVATDGHRLAY
CGVETGEQPAGAGGRHEVIIPRKTILELQRLLEDVDDPVSVQLASNQVKFTFGNIELISK
LVEGKFPDFQRVIPKGYRNSFTIDRAFLQQALQRTAILTTDKFKGVRCMLDTNVLKISST
NADQEEAQEELEIDYQGDALDIGFNVTYLLDVLANLKAEKVQVSLGDSNSSALITLPDDD
TFKYVVMPMRI