Protein Info for UW163_RS03880 in Ralstonia solanacearum UW163
Annotation: Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 96% identical to GATA_RALSO: Glutamyl-tRNA(Gln) amidotransferase subunit A (gatA) from Ralstonia solanacearum (strain GMI1000)
KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 99% identity to rsc:RCFBP_21387)Predicted SEED Role
"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)
MetaCyc Pathways
- L-citrulline biosynthesis (8/8 steps found)
- L-glutamate and L-glutamine biosynthesis (7/7 steps found)
- superpathway of L-citrulline metabolism (10/12 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7
Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (495 amino acids)
>UW163_RS03880 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA (Ralstonia solanacearum UW163) MTASTLKTLSAQLAAKDVSAVELARHYLSRIEARADLNAFVHVDAEATLAQAQAADARIA AGDAGPLAGVPIAHKDVFVTRGWRATAGSKMLDSYVSPFDATVVERLAAAGMVTLGKTNM DEFAMGSSNENSHFGPVKNPWNVGHVPGGSSGGSAAAVAADLAPAATGTDTGGSIRQPAS FSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAHTAEDCALLLSAMAGFDASDSTSLEPG RGGDAEDFGRLLGQPLEGADAARPLAGLRIGLPQEYFGAGLADDVRAAVRAALAELETLG ATLVDISLPKTELSIPTYYVIAPAEASSNLSRFDGVRYGHRAAEYRDLADMYRKSRAEGF GWEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFRNAFQQCDVIMGPVAPTVAWKL GEKTDDPLQMYLADIFTLSTSLAGLPGMSVPAGFGANGLPVGLQIIGNYFEEARMLQIAH AFQQATDWHTRQPAA