Protein Info for UW163_RS03320 in Ralstonia solanacearum UW163

Annotation: glutamine--fructose-6-phosphate transaminase (isomerizing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01135: glutamine-fructose-6-phosphate transaminase (isomerizing)
  • amino acids 2 to 612 (611 residues), 822.1 bits, E=1.5e-251
• PF13522: GATase_6
  • amino acids 64 to 197 (134 residues), 70.9 bits, E=1.6e-23
• PF13537: GATase_7
  • amino acids 87 to 197 (111 residues), 53.2 bits, E=4.3e-18
• PF01380: SIS
  • amino acids 292 to 420 (129 residues), 100.5 bits, E=1e-32
  • amino acids 465 to 594 (130 residues), 80.5 bits, E=1.5e-26

Best Hits

Swiss-Prot: 98% identical to GLMS_RALSO: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 99% identity to rsc:RCFBP_21275)

Predicted SEED Role

"Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.6.1.16)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
• CMP-legionaminate biosynthesis I (2/10 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.16

Protein Sequence (612 amino acids)

>UW163_RS03320 glutamine--fructose-6-phosphate transaminase (isomerizing) (Ralstonia solanacearum UW163)
MCGIVGAVSTRNIVPVLIEGLRRLEYRGYDSCGVAVQRDGQLERARTVSRVADLDTQAQS
SRLDGAIGIAHTRWATHGRPDTVNAHPHFSGDTIALVHNGIIENYETLREELKAVGYGFE
SQTDTEVVAHLIHQAYTYPSSATRGHLFASVRAVVKRLHGAYAIAVFARDNPDVVVGARA
GSPLVVALGDNESFLASDALAVAGTANRIVYLEEGDVVELTREGLTVSDLHDHVVEREVK
VVEAHAAAVELGPYRHFMQKEIFEQPRALGDTLEGVQGFAPDLFGPRAAEVLSKVDSVLI
LACGTSYYSGCTAKYWLESIAKIPTQVEVASEYRYRDTVPNPNALVVVISQSGETADTLA
ALRHARELGHTHTLAICNVATSAMVRETELKFLTRAGTEIGVASTKAFTTQLAALYMLAL
TFAKQRGLLNAQQEEDALRHLRHLPSALNAVLALEPQIIAWSEEFARRENALFLGRGLHF
PIAMEGALKLKEISYIHAEAYPAGELKHGPLALVTEQMPVVTVAPNDALLEKLKSNIQEV
RARGGKLYVFADADTHIQSSDGIQVIRMPEHYGQLSPILHVVPLQLLAYHTAVARGTDVD
KPRNLAKSVTVE