Protein Info for UW163_RS03075 in Ralstonia solanacearum UW163

Annotation: butyryl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 376 PF02771: Acyl-CoA_dh_N" amino acids 4 to 116 (113 residues), 130.9 bits, see alignment E=5.6e-42 PF02770: Acyl-CoA_dh_M" amino acids 120 to 215 (96 residues), 97.7 bits, see alignment E=7.3e-32 PF00441: Acyl-CoA_dh_1" amino acids 227 to 375 (149 residues), 180.9 bits, see alignment E=3.5e-57 PF08028: Acyl-CoA_dh_2" amino acids 242 to 364 (123 residues), 104.1 bits, see alignment E=1.5e-33

Best Hits

Swiss-Prot: 48% identical to ACADS_RAT: Short-chain specific acyl-CoA dehydrogenase, mitochondrial (Acads) from Rattus norvegicus

KEGG orthology group: None (inferred from 100% identity to rsc:RCFBP_21219)

MetaCyc: 64% identical to short-chain acyl-CoA dehydrogenase monomer (Pseudomonas putida KT2440)
RXN-13449 [EC: 1.3.8.1]

Predicted SEED Role

"Isovaleryl-CoA dehydrogenase (EC 1.3.8.4); Butyryl-CoA dehydrogenase (EC 1.3.99.2)" (EC 1.3.8.4, EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.4, 1.3.99.2

Use Curated BLAST to search for 1.3.8.1 or 1.3.8.4 or 1.3.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (376 amino acids)

>UW163_RS03075 butyryl-CoA dehydrogenase (Ralstonia solanacearum UW163)
MLLTPEHEMIRDAIRTFVREAVAPHAAAWDRDATFPKDVHRQLGELGAYGVAVPEALGGA
GLDYLSLALILEEIAAGDGGTSTVVSVNNCPVCSMLMAYANDAQKQQWLVPLARGEMLGA
FCLTEPHVGSDAAALRTTATRDGDHWVLNGVKQFITSGKHADVAIVMAVTDKAAGKRGIS
AFLVPTSTPGYIVARLEDKLGQHSSDTAQIVFEDCRIPADGLLGAQGEGYRMALSGLEGG
RIGIAAQSVGMARAAFEAALAYAKERESFGQPLFAHQAVQFRLAEMATRIEVARQMVWHA
ASLKDAGRPCLKEAAMAKLNASEMAERVCSDAIQVFGGYGYVSDFPVERIYRDVRVCQIY
EGTSDIQKILIARALA