Protein Info for UW163_RS03055 in Ralstonia solanacearum UW163

Annotation: bifunctional isocitrate dehydrogenase kinase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 612 transmembrane" amino acids 331 to 344 (14 residues), see Phobius details PF20423: AceK_regulatory" amino acids 17 to 322 (306 residues), 405.6 bits, see alignment E=1.2e-125 PF06315: AceK_kinase" amino acids 323 to 582 (260 residues), 394.4 bits, see alignment E=2.5e-122

Best Hits

Swiss-Prot: 92% identical to ACEK_RALPJ: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 99% identity to rsc:RCFBP_21215)

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (612 amino acids)

>UW163_RS03055 bifunctional isocitrate dehydrogenase kinase/phosphatase (Ralstonia solanacearum UW163)
MSHFPKLLSSQIAFDVARTMLDGFDKHYRLFREVSVQAKARFESGDWAGLQQLQRDRIAY
YDERVREAAIILEDEYDAENIDDEIWRQIKLHFIGLLTNHHQPECAETFFNSVCTRILHR
SYFNNDFIFVRPAISTEYIENEESPTKPTYRAYYPGSREGLGTCFERIVHNFQLDAPFED
LPRDVGYLVRAVGEHFGDFRIAPNFQIHVLSSLFFRNKAAYVVGRVINGDKTFPLAVPIL
RNAAGQLTLDTVLLRQEQLLILFSFTHSYFMVDMEIPSAYVTFLRDLMPRKPRAEIYTSL
GLQKQGKNLFYRDFLHHLQHSSDKFISAPGIKGLVMLVFTLPSYPYVFKVIKDYFPPPKE
TTRELIKSKYQLVKQHDRVGRMADTLEYSDVAFPLSRFDDELVKELEKHAPSMLEFQRSA
DGGDEIVIRHVYIERRMTPLNIWLHEGTDAQVEHGIVEYGNAIKELIAANIFPGDMLYKN
FGVTRHGRVVFYDYDEIEYLTDCNVREVPQPRNEEEEMSGEVWYSVGPHDIFPQTYRTFL
LGDPRVREAFLRHHADFFDPAMWQAHKERLLSGQIHDFYAYDVSERFVNRYGVGAPANAA
EHTTTALRRAAA