Protein Info for UW163_RS01850 in Ralstonia solanacearum UW163

Annotation: aminoglycoside phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 352 PF01636: APH" amino acids 35 to 270 (236 residues), 134.3 bits, see alignment E=3.2e-43

Best Hits

Swiss-Prot: 56% identical to AMGK_PSEAE: N-acetylmuramate/N-acetylglucosamine kinase (amgK) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K07102, (no description) (inferred from 98% identity to rsc:RCFBP_20967)

MetaCyc: 52% identical to N-acetyl-D-muramate 1-kinase (Pseudomonas putida KT2440)
RXN-18660 [EC: 2.7.1.221]; N-acetylhexosamine 1-kinase. [EC: 2.7.1.221, 2.7.1.162]; N-acetylgalactosamine kinase. [EC: 2.7.1.221, 2.7.1.162, 2.7.1.157]

Predicted SEED Role

"COG3178: Predicted phosphotransferase related to Ser/Thr protein kinases" in subsystem YjeE

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.157 or 2.7.1.162 or 2.7.1.221

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (352 amino acids)

>UW163_RS01850 aminoglycoside phosphotransferase (Ralstonia solanacearum UW163)
MASTPSAAGATNARLAQLRDWLGTLPAAHGLRIDTLRPASADASFRRYFRLDGAAGTLIA
MDAPPPQEDCRPFVHVATLLGGAGVHAPQVLEQDIAQGFLLLTDLGPQTYLQSLRDRDFD
PAYADTLFRPAIDTLVRWQSASREGELPPYDEALLRRELSLFPDWYVERHLQRPLEAAQH
EALDQVFKLLVDSALAQPRVYVHRDYMPRNLMINAADPSQPGVLDFQDAVYGPITYDGAS
LLRDAFLSWEEEQELDWAVRYWEAARRAGLPVGEDFGEFYRALEWMGAQRHLKVAGIFAR
LRYRDGKTGYVEDTPRFIAYLRRVATRYNALAPLARLLDQLEDRATQVGYTF