Protein Info for RPSI07_RS23815 in Ralstonia solanacearum PSI07
Annotation: homoserine O-acetyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 95% identical to METXS_RALSO: Homoserine O-succinyltransferase (metXS) from Ralstonia solanacearum (strain GMI1000)
KEGG orthology group: K00641, homoserine O-acetyltransferase [EC: 2.3.1.31] (inferred from 100% identity to rsl:RPSI07_3352)Predicted SEED Role
"Homoserine O-acetyltransferase (EC 2.3.1.31)" in subsystem Methionine Biosynthesis (EC 2.3.1.31)
MetaCyc Pathways
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
- L-methionine biosynthesis III (4/4 steps found)
- L-homocysteine biosynthesis (2/2 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
- superpathway of L-cysteine biosynthesis (fungi) (3/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.31
Use Curated BLAST to search for 2.3.1.31
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (401 amino acids)
>RPSI07_RS23815 homoserine O-acetyltransferase (Ralstonia solanacearum PSI07) MTELQVDPAAPADQAAAAATHPAATLPPDSVGLVVPERMHFAEPLPLRNGSQIAGYDLMV ETYGTLNAERSNAVLICHALNASHHVAGVHAEGEVGWWDNMVGPAKPVDTDRFFVIGVNN LGSCFGSTGPMSPHPQTGQPYGARFPVITVEDWVNAQARVADRFGIRQFAAVMGGSLGGM QALAWSLMYPERLRHCVVVASTPKLSAQNIAFNEVARSAILSDPDFHGGDYYAHGVKPKR GLRVARMIGHITYLSDEDMAEKFGRELKAEDIRFSFDVEFQVESYLRYQGDKFAEYFDAN TYLLITRALDYFDPALVHGGDLTRAVAHTQAGYLVISFTTDWRFAPARSRELVKALLDNK RPVTYGEIEAPHGHDAFLLEDARYHALVRAYYERIAQEIGA