Protein Info for RPSI07_RS20825 in Ralstonia solanacearum PSI07

Annotation: membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 534 PF02543: Carbam_trans_N" amino acids 98 to 191 (94 residues), 29.8 bits, see alignment E=4.3e-11 amino acids 264 to 308 (45 residues), 43.9 bits, see alignment 2.2e-15 PF16861: Carbam_trans_C" amino acids 357 to 522 (166 residues), 186.8 bits, see alignment E=2.4e-59

Best Hits

KEGG orthology group: K04128, hydroxymethyl cephem carbamoyltransferase [EC: 2.1.3.7] (inferred from 100% identity to rsl:RPSI07_2714)

Predicted SEED Role

"macromolecule metabolism; macromolecule synthesis, modification"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.3.7

Use Curated BLAST to search for 2.1.3.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (534 amino acids)

>RPSI07_RS20825 membrane protein (Ralstonia solanacearum PSI07)
MMRLLAMKPGHDGNLAYIANGRLEFSFEAEKDSGNRYAPIGATNLIEAMRHTPDIPEAIV
ISGWSAGVDPMGRPIGAGYMGLEPPSLSEISLFGQPVKLLSSSHERSHLMGAYALSPFPQ
GQPCYALIWEGHIGAFYSIDASLGIERLAQVMVGPGIRYAFAYGLADPSFGLGRGQIRLS
DAGKLMALAAFEAQVTPSRDEEILLDRMFSDPQGVPFLHKSDFHHLDVYNSGVESEAAKR
LARLVSDRIFDTFFQAIKPLVKEKRPLLIGGGCGLNCEWNRSWLDSGLFSDVFVPPCTND
VGSALGSAADAQWHLTGRAKLDWNVYCGQAFVDDVGYETASRLGPFTRRSTDMADIADTL
RRGEVLGWVTGRAEMGPRALGNRSILAAPFERAMLDRLNTIKRREGFRPIAPVCLEEDVS
THFDLAGPSPYMLYFCKVRSRGSLKAITHVDGSSRAQSVNAKQNPLLYRLLSEFKAQTNF
GVLCNTSLNFNGTGFINRTSDLVRYAQEAGLDGFVVEGVLFNRQRGGSNDMVSA