Protein Info for RPSI07_RS18620 in Ralstonia solanacearum PSI07

Annotation: single-stranded-DNA-specific exonuclease RecJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 TIGR00644: single-stranded-DNA-specific exonuclease RecJ" amino acids 23 to 563 (541 residues), 482.6 bits, see alignment E=6.5e-149 PF01368: DHH" amino acids 74 to 230 (157 residues), 81.4 bits, see alignment E=1e-26 PF02272: DHHA1" amino acids 319 to 449 (131 residues), 87.4 bits, see alignment E=1.9e-28 PF17768: RecJ_OB" amino acids 466 to 563 (98 residues), 68.3 bits, see alignment E=8.5e-23

Best Hits

KEGG orthology group: K07462, single-stranded-DNA-specific exonuclease [EC: 3.1.-.-] (inferred from 100% identity to rsl:RPSI07_2261)

Predicted SEED Role

"Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-)" in subsystem DNA-replication or DNA Repair Base Excision or DNA repair, bacterial RecFOR pathway (EC 3.1.-.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (565 amino acids)

>RPSI07_RS18620 single-stranded-DNA-specific exonuclease RecJ (Ralstonia solanacearum PSI07)
MTRIAIRPHSAETASLLAGHGIHPVLARILAARGVARPSELSTELADLLAPAQLKGIDDA
ARYLADAIAAGKRLLIVADYDCDGATACAVGVRGLRMLGAQVGYIVPNRFEYGYGLSPEI
VALAAREKPDVLVTVDNGIADVAGVAAANALGIDVVVTDHHMPGAQLPEARVIVNPNQPG
CGFPSKHLAGVGVMFYVLLALRAELRNRGAFTLQNQPRLDALLDLVALGTVADVVRLDAN
NRVLVAQGLKRMRAGRMCAGIAALFRVSGREARRASTFDLGFGLGPRLNAAGRLADMSLG
IECLLTDDFDRAMAIAAELDGMNRERREIEAGMQQEAMAILSSTLVGMEGSGSHTIAVYN
ETWHQGVIGIVASRLKDKFHRPVITFAPGDDGVIKGSGRSIPGFHLRDALDAVHKRCPGL
IVKFGGHAMAAGLSLRADGFAPFVETFEAVGREWLNEALLSRVLETDGEADPECFTPQFV
ELLETHVWGQGFPAPSFCGEFDVLSQAVLKEKHLKLQLGRGKQRFDAIWFNHAEPLGASA
YVAYRLDNNTFNGITRVQMVIEHAQ