Protein Info for RPSI07_RS17775 in Ralstonia solanacearum PSI07

Annotation: 2-oxoglutarate dehydrogenase E1 component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 954 PF16078: 2-oxogl_dehyd_N" amino acids 11 to 48 (38 residues), 56.8 bits, see alignment (E = 2.7e-19) TIGR00239: oxoglutarate dehydrogenase (succinyl-transferring), E1 component" amino acids 13 to 943 (931 residues), 1237.6 bits, see alignment E=0 PF00676: E1_dh" amino acids 218 to 522 (305 residues), 153 bits, see alignment E=1.9e-48 PF02779: Transket_pyr" amino acids 600 to 797 (198 residues), 171.5 bits, see alignment E=2.7e-54 PF16870: OxoGdeHyase_C" amino acids 803 to 944 (142 residues), 182 bits, see alignment E=1.3e-57

Best Hits

Swiss-Prot: 88% identical to ODO1_CUPNH: 2-oxoglutarate dehydrogenase E1 component (odhA) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 76% identity to aaa:Acav_2000)

Predicted SEED Role

"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (954 amino acids)

>RPSI07_RS17775 2-oxoglutarate dehydrogenase E1 component (Ralstonia solanacearum PSI07)
MTELYKQYLDTSYLSGGNAAYVEDQYEAYLQDPTSVSEALRAYFDALQNIPAVDGSNARD
IPHAPIVTSFAERAKQGPIKTIVASADSDMGRKRVAATQLVAAYRNVGLRWADLDPLKRQ
ERPPVPDLDPAFYGFTEADQDIVFNASNTYFGKETMSLRELLNNLRETYCGSIGAEFMYI
SDQAQKRWWQERLESIRSKPVFSAEKKKHVLERLTAAEGLERFLHTKYVGQKRFSLEGGE
SFIAAMDELIQHAGEKGVQEIVIGMAHRGRLNVLVNTLGKMPADLFAEFEGKHVDDLPAG
DVKYHKGFSSDVPTPGGPVHLSLAFNPSHLEIVNPVVEGSVKARQERRGDQTGEQEVLAV
QVHGDAAFAGQGVVMETLNLAQTRGYGTGGTIHIVINNQIGFTTSDPRDSRSTLYCTDVV
KMIEAPVLHVNGDDPEAVVLAMQLAIDFRTEFKKDVAVDIICFRKLGHNEQDTPAMTQPL
MYKKIGQHPGTRKLYADKLITQNTLKTEEPDGLVQEYRAAMDAGKHTVDPVLSNFKNKFA
VDWLPFLNRKWTDAADTAVPMAELKRLAERITAIPEHFKVHPLVERVVNDRAKMGQGEQP
LDWGMGEHLAFASLVASGYPVRITGQDAGRGTFTHRHAVLHDQNRERWDAGSYIPLQNVS
DNQAPFNVIDSVLSEEAVMGFEYGYSTAEPNALVIWEAQFGDFANGAQVVIDQFISSGEV
KWGRASGLTLMLPHGYEGQGPEHSSARLERYLQLCADHNMQVVQPTTPAQIFHLLRRQMI
RLFRKPLIILTPKSLLRSKDAVSPLTDLAKGHFETVIADAEELNAAKVKRVVACSGKVYY
DLVNSRKERGQTDTAIIRVEQLYPFPHKAFAAELKKYPNLTEVVWCQDEPQNQGAWFFVQ
HYIMENMSEGQKLGYAGRPASASPAVGYYAKHNEQQKALVDAAFTKLKGFVLTK