Protein Info for RPSI07_RS17185 in Ralstonia solanacearum PSI07

Annotation: 50S ribosomal protein L3 N(5)-glutamine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 TIGR03533: protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific" amino acids 13 to 297 (285 residues), 408.5 bits, see alignment E=1.5e-126 TIGR00536: methyltransferase, HemK family" amino acids 14 to 272 (259 residues), 206.5 bits, see alignment E=4.6e-65 PF06325: PrmA" amino acids 134 to 209 (76 residues), 24.5 bits, see alignment E=5.3e-09 PF13847: Methyltransf_31" amino acids 134 to 266 (133 residues), 29.3 bits, see alignment E=2e-10 PF05175: MTS" amino acids 135 to 217 (83 residues), 46 bits, see alignment E=1.4e-15 PF13649: Methyltransf_25" amino acids 137 to 214 (78 residues), 33.4 bits, see alignment E=1.8e-11 PF01170: UPF0020" amino acids 143 to 216 (74 residues), 23.7 bits, see alignment E=1e-08

Best Hits

Swiss-Prot: 71% identical to PRMB_BURPS: 50S ribosomal protein L3 glutamine methyltransferase (prmB) from Burkholderia pseudomallei (strain K96243)

KEGG orthology group: K07320, putative adenine-specific DNA-methyltransferase [EC: 2.1.1.72] (inferred from 100% identity to rsl:RPSI07_1975)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p"

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (300 amino acids)

>RPSI07_RS17185 50S ribosomal protein L3 N(5)-glutamine methyltransferase (Ralstonia solanacearum PSI07)
MTVSALPASHPFTTVRDLLRYAVSRFTAAGLVFGHGSENAYDEAAYLILHTLHLPLDTLE
PFLDARLLPEEVAAVLQVIERRTVDRVPAAYLTHEAYMHGMRFYVDERVIVPRSFIGELL
EDGLAPWLPYEDGPTDVLELCTGSGCLAILAALQWPNATLDAVDLSPDALVVAQRNVDDY
HLNDRIRLHEGDLYAPLPPGVHYDVILTNPPYVNETSMQALPPEYRAEPRMALAGGTDGM
DIVRRILADAPRHLKPHGVLVVEIGNERENVEAAFPDLDLVWLPTSAGEEQVFLVTREAL