Protein Info for RPSI07_RS17110 in Ralstonia solanacearum PSI07

Annotation: [protein-PII] uridylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 861 TIGR01693: protein-P-II uridylyltransferase" amino acids 23 to 859 (837 residues), 794.1 bits, see alignment E=8.9e-243 PF03445: DUF294" amino acids 23 to 106 (84 residues), 24.7 bits, see alignment E=5.6e-09 PF01909: NTP_transf_2" amino acids 61 to 96 (36 residues), 24 bits, see alignment (E = 1.2e-08) PF08335: GlnD_UR_UTase" amino acids 166 to 304 (139 residues), 140.4 bits, see alignment E=1.3e-44 PF01966: HD" amino acids 442 to 536 (95 residues), 34.7 bits, see alignment E=5.8e-12

Best Hits

Swiss-Prot: 96% identical to GLND_RALSO: Bifunctional uridylyltransferase/uridylyl-removing enzyme (glnD) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00990, [protein-PII] uridylyltransferase [EC: 2.7.7.59] (inferred from 68% identity to bac:BamMC406_1923)

Predicted SEED Role

"[Protein-PII] uridylyltransferase (EC 2.7.7.59)" in subsystem Ammonia assimilation (EC 2.7.7.59)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (861 amino acids)

>RPSI07_RS17110 [protein-PII] uridylyltransferase (Ralstonia solanacearum PSI07)
MHTAAAATPVLSPRDILKAERAHLFAQFEQHANVNQLATKLARAVDQALTQLWRDEGMPD
DCALIAVGGYGRGELFPYSDVDILLLLPQAADKALEARLEAFIGRCWDMGLDIGSSVRTV
DECISEAEQDVTVRTSLLEARLLTGDEGLYRTFETHYQGHLDAADFYQSKMLEMRQRHAK
YQDTPYSLEPNCKESPGGLRDLQVILWMTRAAGFGSSWNELLVNQLLTRREAKELAANER
LLKTIRARLHLLAGRRQDVLVFDLQTQLGEAFGYRPNAAKRASEQLMRRYYWAAKAVTQL
NTVVLQNIEARLFPTELGITRTINERFIERQGTLEIADPDLYQREPAAILETFLVYEQTR
GVKGLAANTLRALYNARTLMDAKWRNDPANRATFLSILQQPQGITHALRLMNQTSVLGRY
LVNFRRIVGQMQHDLFHVYTVDQHILMVVRNVRRFAIVEHAHEFPFCSQLMANFDKPWVL
TIAALFHDIAKGRGGDHSVLGMTDARRFCKQHNLASEDADLIVWLVEHHLTMSQVAQKQD
LGDPEVIRHFADLVGSERYLTALYLLTVADIRGTSPKVWNAWKAKLLEDLYRITLRVLGG
ATTDPHAVLESRKEEARTLLRLAAMDPHVHEALWAQLDVGVFLRHDARDIAWFTRHFYNR
VDTTIPIVRARISPVGEGLQVAVYSPDRPDLFARICGYFERKGLTILDAKIHTTKHGYAL
DTFQVADPGTGLVEPGHYRDIITLVEHELAELIAREAALPEPPRGRISRQSRSFPIKPRV
DLRPDERGQYYLLSLSATDRTGLLYAIARVLARHRVSVHTARINTLGERVEDVFLLDGSR
LTQDNKLQLALESELLEALAV