Protein Info for RPSI07_RS15330 in Ralstonia solanacearum PSI07

Annotation: polyphosphate kinase 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 724 TIGR03705: polyphosphate kinase 1" amino acids 9 to 688 (680 residues), 971.5 bits, see alignment E=1.2e-296 PF13089: PP_kinase_N" amino acids 10 to 114 (105 residues), 117.8 bits, see alignment E=7e-38 PF02503: PP_kinase" amino acids 124 to 305 (182 residues), 195.3 bits, see alignment E=1.9e-61 PF17941: PP_kinase_C_1" amino acids 333 to 503 (171 residues), 243.7 bits, see alignment E=1.9e-76 PF13090: PP_kinase_C" amino acids 514 to 681 (168 residues), 212.1 bits, see alignment E=9.9e-67

Best Hits

Swiss-Prot: 70% identical to PPK1_BURM1: Polyphosphate kinase (ppk) from Burkholderia multivorans (strain ATCC 17616 / 249)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 100% identity to rsl:RPSI07_1577)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (724 amino acids)

>RPSI07_RS15330 polyphosphate kinase 1 (Ralstonia solanacearum PSI07)
MSTLPTGDLLNREVGILEFNARVLAQAADPGVPLMERLKFICIVSSNLDEFFEIRVAGLK
EQMRDNPSALTPDGLSVQQAFGVVTERVRQLVAQQYAMLQDEILPLLEKEGVFFHMTTNW
NEAQRAWCRSFFQRELVPILTPMALDPAHPFPRVLNKSLNFIIELSGKDAFGREAELAIV
QAPRALPRLVQMPPELSGYPHGFVLLSSFMQGFVHELFPQIDVHGCYQFRVTRNSDLFVS
EDEITDLREALQGELSTRHFGDAVRLEIAHDISPALARRLQQEFGLSEADCYRVKGPVNL
VRLLQVPDLVDRPVLKYPPHAPAAVKAVTASSSIFDAIRQGDILMHHPYESFTPVLELLQ
QAARDPDVVAIKQTVYRTGNESSVMDALIKAARNGKEVTVVVELLARFDEETNINWADQL
ESAGAHVVYGVVGHKCHAKMLLVVRREAAGGKAKAVRLRRYAHLGTGNYHPKTARLYTDF
GLMTANETICEDVHHVFQLLTGTGAQVKLHHLWHSPFTMHANLVEHIRAEARAARAGKRA
RIIGKMNALLEPTIIEELYKASRAGVKIDLIVRGVCALRAGVPGLSEHISVRSIVGRFLE
HHRVYYFHAGGEEVVYLSSADWMDRNLFRRVEVAFPVLDRKMKARVIRESLQVHLRDNAS
AWVMQPDGTYVRRQSKGKHVRVSQLDLLNHFSPQAAAAAETAAAVAAATATEPTLKPPAE
ISAG