Protein Info for RPSI07_RS12825 in Ralstonia solanacearum PSI07

Annotation: leucyl aminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 506 PF02789: Peptidase_M17_N" amino acids 27 to 148 (122 residues), 101 bits, see alignment E=4.9e-33 PF00883: Peptidase_M17" amino acids 197 to 497 (301 residues), 432.1 bits, see alignment E=1.3e-133

Best Hits

Swiss-Prot: 95% identical to AMPA_RALSO: Probable cytosol aminopeptidase (pepA) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 100% identity to rsl:RPSI07_1059)

Predicted SEED Role

"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (506 amino acids)

>RPSI07_RS12825 leucyl aminopeptidase (Ralstonia solanacearum PSI07)
MEFSTKALDWAKAGPSGALAAKSDCLVIGLFESQTLAGAAKALDVATKGLVGRLVKLGDF
EGKRGTSLLLHEVAGVGAARVLLVGLGKEAEFTDRAYAEAVRTALRALSGTKATNVTWTL
TQHAARDKDAAWAVLTAVTLIREASYRFIERHPELKSKRDKSGSGLRKVVLTVDAADAKT
AAVAAARGTAIANGMDLTRDLGNLPSNICTPTYLANTARQIAKAFKLKVEVLGRKQIEAL
KMGAFLAVTKGSEEPPQFIVLRHEGGPAKQAPVVLVGKGVTFDTGGISLKPGEGMDEMKY
DMCGAASVLGTLRAVAEMGLKQNVIAVVPTCENMPSGIATKPGDVVTSMSGQTIEVLNTD
AEGRLILCDALTYVERFKPAAVIDVATLTGACIIALGHVNTGMFARSDTLANALLAAGKQ
SLDTAWRMPLDEEYQEQLKSNFADMGNIGGRPAGSVTAACFLARFTEKYDWVHLDIAGTA
WKSGAAKGATGRPVPLLTRFLMDRAG