Protein Info for RPSI07_RS12645 in Ralstonia solanacearum PSI07

Annotation: aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00487: FA_desaturase
  • amino acids 18 to 233 (216 residues), 56.5 bits, E=3.7e-19
• PF01610: DDE_Tnp_ISL3
  • amino acids 273 to 395 (123 residues), 33.3 bits, E=4.4e-12

Best Hits

KEGG orthology group: K00507, stearoyl-CoA desaturase (delta-9 desaturase) [EC: 1.14.19.1] (inferred from 100% identity to rsl:RPSI07_1024)

Predicted SEED Role

"Fatty acid desaturase (EC 1.14.19.1); Delta-9 fatty acid desaturase (EC 1.14.19.1)" (EC 1.14.19.1)

MetaCyc Pathways

• oleate biosynthesis II (animals and fungi) (2/2 steps found)
• palmitoleate biosynthesis IV (fungi and animals) (2/2 steps found)
• sorgoleone biosynthesis (2/8 steps found)

KEGG Metabolic Maps

• Biosynthesis of unsaturated fatty acids

Isozymes

Compare fitness of predicted isozymes for: 1.14.19.1

Use Curated BLAST to search for 1.14.19.1

Protein Sequence (396 amino acids)

>RPSI07_RS12645 aminotransferase (Ralstonia solanacearum PSI07)
MIDSFLSFLANGTLNWAWWQILIFTLVVTHITITSVTIFLHRCQAHRALDLHPAAAHVFR
FWLWLSTGMVTKEWAAIHRKHHAKCETEEDPHSPQTRGIRKVLLEGAELYRAEAKNRETI
QKFGHGTPNDWIEQNLYSKYTWQGVGLMLILDVLMFGAVGLTVWAVQMLWIPVNAAGIIN
GLGHYWGYRNFDCEDASTNVFPWGLIIGGEELHNNHHTYATSAKFSIKWYEFDVGWGYIR
ALEMVGLAKVKKVAPKARFGDVKPVDQNTLEAIIANRYDVMARYAKTVKAAYRQELAKLK
AARDVEYRALKPARKWFHRDEAKLGAPQREQLSQIVSDHSALHTFVEMRRDLGALWGRSN
ATREQLLHQLQEWCKRAEESGIHALQEFALRLRRYA