Protein Info for RPSI07_RS12000 in Ralstonia solanacearum PSI07

Annotation: polynucleotide adenylyltransferase PcnB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 515 TIGR01942: poly(A) polymerase" amino acids 54 to 498 (445 residues), 485.1 bits, see alignment E=9.1e-150 PF01743: PolyA_pol" amino acids 84 to 233 (150 residues), 118 bits, see alignment E=5.3e-38 PF12627: PolyA_pol_RNAbd" amino acids 260 to 322 (63 residues), 74.9 bits, see alignment E=5.4e-25 PF12626: PolyA_pol_arg_C" amino acids 374 to 496 (123 residues), 151.7 bits, see alignment E=1.3e-48

Best Hits

KEGG orthology group: K00970, poly(A) polymerase [EC: 2.7.7.19] (inferred from 100% identity to rsl:RPSI07_0890)

Predicted SEED Role

"Poly(A) polymerase (EC 2.7.7.19)" in subsystem Polyadenylation bacterial (EC 2.7.7.19)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (515 amino acids)

>RPSI07_RS12000 polynucleotide adenylyltransferase PcnB (Ralstonia solanacearum PSI07)
MIKKLINRLLGRPGAETAHATEPGTPAKKGRAPKTPKSRAKAAAGGVPRAPRVWNVEAHG
IDPKLLSRNAVKVTSTLQEAGYDAFIVGGAVRDLLLGIQPKDFDVATNATPEQVEALFRR
SRIIGRRFQIVHVTFYGGREQEIIEVSTFRALVDAVESEQVPAGKRVKRTELDHHTHAVD
ASGRVLRDNVWGTQAEDATRRDFTVNAMYYNPADQTVHDYHHGMEDMRARLLRMIGDPHT
RYREDPVRMLRVVRFAAKTGFDIDPATRAPIAELGPLIHNVPAARLFDEMLKLLMSGHAW
ASLQELRKAGLHSGLLPLLDVALEQPMGQRFVQLALDNTDERVKAGKPVSPGFLFASLMW
HHVLEHWNRRRAAGEPLIPALHTAMDEVLDRQTEQLAIQRRFTADMKEIWSMQPRFEKRT
GRMPYRLLELPRFRAGYDFLALRCASGELPQELATWWTDFQNGDGDAREALIAQAKQAPA
AAGGSTGGVKRRRRRRGPAKSDTVADVSPGSSEGT