Protein Info for RPSI07_RS11925 in Ralstonia solanacearum PSI07

Annotation: imidazolonepropionase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 transmembrane" amino acids 327 to 344 (18 residues), see Phobius details amino acids 357 to 379 (23 residues), see Phobius details TIGR01224: imidazolonepropionase" amino acids 30 to 404 (375 residues), 469 bits, see alignment E=5e-145 PF01979: Amidohydro_1" amino acids 66 to 404 (339 residues), 68.6 bits, see alignment E=6.1e-23 PF07969: Amidohydro_3" amino acids 114 to 385 (272 residues), 51 bits, see alignment E=1.7e-17

Best Hits

Swiss-Prot: 97% identical to HUTI_RALSO: Imidazolonepropionase (hutI) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01468, imidazolonepropionase [EC: 3.5.2.7] (inferred from 100% identity to rsl:RPSI07_0873)

MetaCyc: 62% identical to imidazolonepropionase (Pseudomonas fluorescens)
Imidazolonepropionase. [EC: 3.5.2.7]

Predicted SEED Role

"Imidazolonepropionase (EC 3.5.2.7)" in subsystem Histidine Degradation (EC 3.5.2.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (408 amino acids)

>RPSI07_RS11925 imidazolonepropionase (Ralstonia solanacearum PSI07)
MADIRWDALWTHVHLATLAADADGYGEILDGAIAVKDGRIAWLGARADLPAGARAEREHD
GGGAWLTPGLIDCHTHLVYAGNRSNEFEARLNGVAYEHIARAGGGILSTVRATRAVSEDA
LAEASLPRLNALRAEGVTTVEIKSGYGLDLETERRMLRVARRFSQTLPVRVRTTFLGAHA
VPPEFAGRADDYIGHLCADVLPALVAEGLVDAVDAFCETIGFSPAQTARMFDAAQALGLP
IKLHAEQLSDQGGAALVARYGGLSADHLECLTDAGVAAMAEAGTVAVLLPGAFYCLRETR
LPPVDALRQAGVPMAVSTDCNPGTSPLSSLLLAMNMACTLFRLTPLEALTGATRHAAAAL
GLAGTCGALAPGCVADFALWRIDRPADLAYAMGLNPCAGVVKDGVPAS