Protein Info for RPSI07_RS11625 in Ralstonia solanacearum PSI07

Annotation: DNA helicase RecG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 729 PF17191: RecG_wedge" amino acids 47 to 194 (148 residues), 35.7 bits, see alignment E=1.7e-12 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 57 to 700 (644 residues), 692.2 bits, see alignment E=4e-212 PF04851: ResIII" amino acids 299 to 467 (169 residues), 30.5 bits, see alignment E=8.1e-11 PF00270: DEAD" amino acids 303 to 473 (171 residues), 80.3 bits, see alignment E=3.7e-26 PF00271: Helicase_C" amino acids 512 to 623 (112 residues), 61.9 bits, see alignment E=1.7e-20 PF19833: RecG_dom3_C" amino acids 652 to 711 (60 residues), 37.1 bits, see alignment 6.8e-13

Best Hits

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 100% identity to rsl:RPSI07_0800)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (729 amino acids)

>RPSI07_RS11625 DNA helicase RecG (Ralstonia solanacearum PSI07)
MPPRARSATAAVPAPDSDAAPAVGPKRAPAAKPADKPAAKTARPADKLAKLGLRRDVDLV
LHLPMRYEDETTLLTIAEAVARANTGWAAQVEGGVTRNEVALRPRRQLVVHIADDSGELV
LRFLNFYGSQVKQMAEGVRLRVRGEVRGGFFGAEMVHPTVRAVAPDEPLPDRLTPVYPST
AGVAQAYLRKAILNALGRTPLPETLPDNLMRGPLAPLKLMAPAEAVRLLHQPTPDVDEHS
LIERTHPAWLRIKFDELLAQQLSLKRAQAARRTRSAPVLRTGGADGLLARFMAALPFKLT
GAQARVWEEIRADLARPYPMQRLLQGDVGSGKTVIAALAACQAIDAGWQAALMAPTELLA
EQHYRKLSAWLEPLGVEIVWLAGSLKRKQKDEAAARVAAGAAQLVIGTHALIQDTVAFAR
LGLAVVDEQHRFGVAQRLALRGKAGGADTPIAETAQVPHQLMMSATPIPRTLAMTYYADL
DVSAIDELPPGRTPVVTRLVNDARRDEVIERIHAAAREGRQVYWVCPLIEESEALQLQTA
VETFETLSQSLAGLKVGLVHGRLPSVDKAAVMSAFAGGELHVLVATTVIEVGVDVPNASL
MVIEHAERFGLAQLHQLRGRVGRGTAESVCILLYQAPLSPTAKQRLQTMRETTDGFEIAR
RDLDIRGPGEFLGARQSGEAMLRFADLNHDAWMVEFAQGAAEQMLARFPEAVEAHLKRWL
GEREHYLRV