Protein Info for RPSI07_RS10650 in Ralstonia solanacearum PSI07

Annotation: glutamyl-tRNA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 transmembrane" amino acids 257 to 274 (18 residues), see Phobius details TIGR01035: glutamyl-tRNA reductase" amino acids 4 to 427 (424 residues), 410.4 bits, see alignment E=4.3e-127 PF05201: GlutR_N" amino acids 6 to 163 (158 residues), 179.6 bits, see alignment E=6.9e-57 PF01488: Shikimate_DH" amino acids 179 to 317 (139 residues), 148.9 bits, see alignment E=2e-47 PF00745: GlutR_dimer" amino acids 332 to 427 (96 residues), 95.1 bits, see alignment E=5.5e-31

Best Hits

Swiss-Prot: 99% identical to HEM1_RALSO: Glutamyl-tRNA reductase (hemA) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K02492, glutamyl-tRNA reductase [EC: 1.2.1.70] (inferred from 100% identity to rsl:RPSI07_0602)

Predicted SEED Role

"Glutamyl-tRNA reductase (EC 1.2.1.70)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.70

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (435 amino acids)

>RPSI07_RS10650 glutamyl-tRNA reductase (Ralstonia solanacearum PSI07)
MQLLTLGINHHTAPLSLREKVAFPLEQLKPALGTLRQHLPHRKSTAEAAILSTCNRTELY
CATDGNLPEDTAHEHAIRWLAHHHNIDASELAPHVYALGQSQAVRHAFRVASGLDSMVLG
ETQILGQMKDAVRTATEAGALGTYLNQLFQRTFAVAKEVRGTTEIGAHSVSMAAAAVRLA
QRIFESITEQRVLFIGAGEMIELCATHFAAQKPREAVVANRTLERGEKLAEALSNQGLNA
RAVHLAELPARLGEFDIVVSCTASTLPIIGLGAVERAIKQRRHRPMFMVDLAVPRDIEPE
VARLSDVFLYTVDDLGAIVREGNALRQAAVAQAEAIIETRVQNFMHWLDARSVVPVIRDL
HAHAEALRQGELERAQRMLARGDDPALVLEALSQALTKKFLHGPTHALNHAQGEGREQLI
DLLPGLFRQSSHSER