Protein Info for RPSI07_RS10320 in Ralstonia solanacearum PSI07
Annotation: glutamate synthase subunit alpha
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00265, glutamate synthase (NADPH/NADH) large chain [EC: 1.4.1.13 1.4.1.14] (inferred from 76% identity to aaa:Acav_0979)Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-citrulline biosynthesis (8/8 steps found)
- L-glutamate and L-glutamine biosynthesis (7/7 steps found)
- superpathway of L-citrulline metabolism (10/12 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of ammonia assimilation (plants) (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle I (2/2 steps found)
- L-glutamate biosynthesis IV (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14
Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1583 amino acids)
>RPSI07_RS10320 glutamate synthase subunit alpha (Ralstonia solanacearum PSI07) MEQHSSPAAPQSAESTTLRGVPSAQGLYDPSNEHDACGVGFVAHIKGKKSHEIVQQGLRI LHNLDHRGAVGADPLMGDGAGILLQIPDQYYREEMARQGVHLPPAGEYGVGMIFLPKEHA SRLACEQELERTVRLEGQVVLGWRDVPVDKTMPMSPTVQKTEPVIRQIFIGRGRDIMTTD ALERKLYVIRKTASHAIQALKLKHGKEYFVPSMSARTVVYKGLLLCEQVGRYYQDLADPR TVSALALVHQRFSTNTFPAWELAHPYRMVAHNGEINTVKGNVNWINARTGGISSPVLGDD LHKLWPLIYPGQSDTASFDNCLELLTMAGYPLAQAMMMMIPEAWEQHTLMDDNRRAFYEY HAAMMEPWDGPAAICFTDGRQIGATLDRNGLRPARYYVTDDDMVVMASEAGVLPIPESRI VKKWRLQPGKMFLIDMEQGRIIDDKELKDNLANAKPYKSWIDAVRIKLDELEAKPEDVAA ETKPAAKLLDRQQSFGYTQEDVKFLMAPMAANGEEATGSMGNDSPLAVLSSKNKTLYHYF KQLFAQVTNPPIDPIRENMVMSLVSFIGPKPNLLELNNINPPMRLEVSQPVLDFKDMAKI RNIEHYTGGKFMAYELNICYPVAWGKEGIEARLASLCAEAVDAVKSGYNILIVSDRAVDE KQAAIPALLATSAIHHHLVEKGLRTSTGLVVETGSAREVHHFALLAGYGAEAVHPYLAME TLAEMAPSLGDLSPEKAVKNFVKAIGKGLQKVMSKMGISTYMSYTGAQIFEAIGLSRELV DKYFHGTASNVGGIGIFEVAEEALRLHRDAFGDAPVLANMLEAGGEYAFRIRGEEHMWTP DSIAKLQHATRANSYQTYKEYANLINDQSKRHMTLRGLFEFKVEPARAIALDEVEPAKEI VKRFATGAMSLGSISTEAHTTLAVAMNRIGGKSNTGEGGEDERRYRNELRGIPIKQGTKL SDVIGRDVIERDLELQEGDSLRSKIKQVASGRFGVTAEYLASADQIQIKMAQGAKPGEGG QLPGHKVTDYIGKLRYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPRSDVSVKLVS EVGVGTVAAGVAKAKADHVVIAGHDGGTGASPWSSIKHAGTPWELGLAETQQTLMLNGLR NRIRVQADGQMKTGRDVVIGALLGADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDP VLRKKFSGRPEHVVNYFFFVAEEVREIMAQLGIRSFSELIGRADLLDTKAGIEHWKARGL DFARIFHQPPKKEGQPCYQVDVQDHGLDRALDHQLIEKARAAIDKGERVSFIQPVRNVNR TVGAMLSGEVAKRYGHEGLPDDSIHIQLQGTAGQSFGAFLAHGVTLDLVGDGNDYVGKGL SGGRVIVRPAHEFRGDPTANIIVGNTVLYGALAGEAFFNGVAGERFAVRNSGATAVVEGT GDHGCEYMTGGTVVVLGATGRNFAAGMSGGVAYVYDEDGLFDKRCNTAMVALESVLSAAD QEKAHTPSTWHKAGGERVLDEQLLKALVEKHFRYTGSERAKELLADWNNARRRFVKVFPT EYKRALTEMYEREQESAREQIAA