Protein Info for RPSI07_RS08600 in Ralstonia solanacearum PSI07

Annotation: ATP-dependent DNA helicase Rep

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 705 PF00580: UvrD-helicase" amino acids 9 to 284 (276 residues), 277.6 bits, see alignment E=3.3e-86 PF13245: AAA_19" amino acids 21 to 267 (247 residues), 78.2 bits, see alignment E=1.7e-25 PF13361: UvrD_C" amino acids 289 to 439 (151 residues), 127.3 bits, see alignment E=2.5e-40 amino acids 511 to 649 (139 residues), 105.5 bits, see alignment E=1e-33 PF13538: UvrD_C_2" amino acids 584 to 644 (61 residues), 31.9 bits, see alignment 2.5e-11

Best Hits

KEGG orthology group: K03656, ATP-dependent DNA helicase Rep [EC: 3.6.4.12] (inferred from 100% identity to rsl:RPSI07_0155)

Predicted SEED Role

"ATP-dependent DNA helicase Rep" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (705 amino acids)

>RPSI07_RS08600 ATP-dependent DNA helicase Rep (Ralstonia solanacearum PSI07)
MSSGLAHGLNAAQSEGVHYLDGPCLVLAGAGSGKTRVITQKIAHLILDKGFEPRNIAAVT
FTNKAAKEMQERVAKLMDGKTREDGKRIPIKQLTVCTFHSLGVQILRAEAEHVGLKPRFS
IMDSDDCFGMIQEQLATTDKQLIRRVQSAISLWKNGLVDPEAAIAEAIANNNADDHQAAL
VYRNYVATLHAYQAVDFDDLIRIPAELFASNEAVRLKWQNRLRYFLVDEYQDTNACQYQL
LKLLAGGSHLRAPAFTAVGDDDQAIYGWRGATLDNLKLLQTDFPNLKVIKLEQNYRSTVR
ILNAANAVIAQNPKLFEKTLWSEHGMGDAVNVAGANDEEHEAESVVFKLSAHKFERRAQF
RDYAILYRGNFQARLFEQILRRERIPYVLSGGQSFFDKAEIKDLCAYLRLIANADDDPAF
IRAVTTPKRGVGNATLEVLGAFAGQAKVSLFEAAMMGGIEAKLAPRQHEPLRVFCEFIVR
LSDRAAREPASTLLDEMMGGIHYEAYLYDTYDERQAQNKWTNVLEFLDWLKRKGTKPENL
EAEDATGYDNADGLMDTGKNLLELTQTIALISMLEGKEEDPDAVRLSTLHASKGLEYPHV
FLVGVEEGILPHCREDDDLTDEKIEEERRLMYVGITRAQRSLHISWCKKRKRARETVVCE
PSRFIAEMKLDDGAGPTPEDTMPAMSPKDRLGALKGLLAAKKPAA